CHIR 99021 trihydrochloride

Name: CHIR 99021 trihydrochloride
Brand: Tocris Bioscience
SKU: 4953

Tocris Bioscience, part of Bio-Techne | Catalog # 4953

Citations (34)

COA / SDS

Hydrochloride salt of CHIR 99021 (Cat. No. 4423); selective GSK-3 inhibitor

Key Product Details

Description:	Hydrochloride salt of CHIR 99021 (Cat. No. 4423); selective GSK-3 inhibitor
Alternative Names:	CT99021
Chemical Name:	6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile trihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	574.72

View Full Technical Details for CHIR 99021 trihydrochloride

Technical Data
Citations

Biological Activity for CHIR 99021 trihydrochloride

CHIR 99021 trihydrochloride is a hydrochloride salt of CHIR 99021 (Cat. No. 4423), a highly selective GSK-3 inhibitor.

CHIR 99021, CHIR 99021 in solution and CHIR 99021 synthesized to cGMP guidelines also available.

External Portal Information

Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of CHIR 99021 is reviewed on the chemical probes website.

Technical Data for CHIR 99021 trihydrochloride

M. Wt	574.72
Formula	C₂₂H₁₈Cl₂N₈.3HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1782235-14-6
PubChem ID	78243722
InChi Key	DSFVSCNMMZRCIA-UHFFFAOYSA-N
Smiles	ClC(C=C3Cl)=CC=C3C1=NC(NCCNC4=CC=C(C#N)C=N4)=NC=C1C2=NC(C)=CN2.Cl.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for CHIR 99021 trihydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	14.37	25
	DMSO	28.74	50

Preparing Stock Solutions for CHIR 99021 trihydrochloride

The following data is based on the product molecular weight 574.72.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	3.48 mL	17.40 mL	34.80 mL
2.5 mM	0.70 mL	3.48 mL	6.96 mL
5 mM	0.35 mL	1.74 mL	3.48 mL
25 mM	0.07 mL	0.35 mL	0.70 mL

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References for CHIR 99021 trihydrochloride

References are publications that support the biological activity of the product.

Ring Selective glycogen synthase kinase 3 inhibitors potentiate Ins activation of glucose transport and utilization in vitro and in vivo. Diabetes 2003 PMID: 12606497
Ye Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embyronic stem cells from refractory mouse strains. PLoS One 2012 PMID: 22540008
Pan AKT kinase activity is required for Li to modulate mood-related behaviors in mice. Neuropsychopharmacology 2011 PMID: 21389981