CRT 0066854 hydrochloride

Name: CRT 0066854 hydrochloride
Brand: Tocris Bioscience
SKU: 5922

Tocris Bioscience, part of Bio-Techne | Catalog # 5922

Citations (2)

COA / SDS

PKCι and PKCζ inhibitor

Key Product Details

Description:	PKCι and PKCζ inhibitor
Chemical Name:	(2S)-3-Phenyl-N¹-[5,6,7,8-tetrahydro-2-(4-pyridinyl)[1]benzothieno[2,3-d]pyrimidin-4-yl]-1,2-propanediamine dihydrochloride
Purity:	≥95% (HPLC)
Molecular Weight:	488.48

View Full Technical Details for CRT 0066854 hydrochloride

Technical Data
Citations

Biological Activity for CRT 0066854 hydrochloride

CRT 0066854 hydrochloride is a protein kinase C (PKC) inhibitor. Inhibits PKCι and PKCζ (IC₅₀ values are 132 and 639 nM, respectively). Restores polarized morphogenesis in oncogene expressing MDCK cells, decreases colony formation in HeLa cells and inhibits lethal giant larvae 2 (LLGL2) phosphorylation.

Compound Libraries for CRT 0066854 hydrochloride

CRT 0066854 hydrochloride is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for CRT 0066854 hydrochloride

M. Wt	488.48
Formula	C₂₄H₂₅N₅S.2HCl
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	2250019-91-9
PubChem ID	131954580
InChi Key	NYUVOWXZRBZQBA-NTEVMMBTSA-N
Smiles	N[C@@H](CC1=CC=CC=C1)CNC2=C3C4=C(SC3=NC(C5=CC=NC=C5)=N2)CCCC4.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for CRT 0066854 hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	48.85	100
	DMSO	48.85	100

Preparing Stock Solutions for CRT 0066854 hydrochloride

The following data is based on the product molecular weight 488.48.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.05 mL	10.24 mL	20.47 mL
5 mM	0.41 mL	2.05 mL	4.09 mL
10 mM	0.20 mL	1.02 mL	2.05 mL
50 mM	0.04 mL	0.20 mL	0.41 mL

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References for CRT 0066854 hydrochloride

References are publications that support the biological activity of the product.

Kjaer Adenosine-binding motif mimicry and cellular effects of a thieno[2,3-d]pyrimidine-based chemical inhibitor of atypical protein kinase C isoenzymes. Biochem.J. 2013 PMID: 23418854
Linch Regulation of polarized morphogenesis by protein kinase C iota in oncogenic epithelial spheroids. Carcinogenesis 2014 PMID: 24072773