Cy5 Labeled Hyaluronan (Low MW)

Applications

• Studying hyaluronan protein binding.
• Used as a substrate for various hyaluronan specific hydrolases and lyases.

Key Features and Benefits:

• Excitation at 649 nm and emission at 671 nm.
• The fluorescent dye Cy5 is conjugated to the non-reducing end GlcNAc residue through enzymatic conjugation.
• Can be separated on 15%-17% SDS-PAGE and directly visualized as a ladder containing 5-100 repeating units (RU) of the HA disaccharide GlcA-β,3-GlcNAc.
• Linear response range for Cy5 labeled glycans can be from 10 fmol to 100 pmol, depending on the sensitivity of detection.
• Store at

For Details:

Wu et al., (2019) Glycobiology 29:750-754
Wu et al., (2020) Glycobiology 30:970-980

Related Reagents

Click Chemistry

• GDP-Cy5-Fucose (ES301)
• CMP-Cy5-Sialic Acid (ES302)
• GDP-Cy3-Fucose (ES401)
• CMP-Cy3-Sialic Acid (ES402)

Enzymes and detection reagents

• Various Hyaluronidases and Hyaluronan Lyase
• HA-binding Proteins
• Hyaluronan

Data Examples

Hyaluronan, also known as hyaluronic acid (HA) or sodium hyaluronate, is a naturally occurring linear polymer. Hyaluronan is a glycosaminoglycan (GAG) that is ubiquitously present in the extracellular matrix of all vertebrates and is also present in the capsule of some strains of Streptococci. Mammalian hyaluronan is synthesized by one of three distinct hyaluronan synthases (HAS1, 2, and 3), which produce HA polymers with different chain lengths and differ in their rates of synthesis. Whereas high molecular weight HA (> 500 kDa) is anti-angiogenic, anti-inflammatory and immunosuppressive, low molecular weight HA (10 - 500 kDa) is highly angiogenic and pro-inflammatory. HA oligomers are anti-apoptotic and upregulate heat shock protein expression. Functionally, hyaluronan molecules are important for the maintenance of a highly hydrated extracellular matrix in tissues, which is involved in cell adhesion and supports cell migration.