Cyclopamine

Name: Cyclopamine
Brand: Tocris Bioscience
SKU: 1623
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1623

(1)

Citations (70)

COA / SDS

Inhibitor of Hedgehog (Hh) signaling

Key Product Details

Description:	Inhibitor of Hedgehog (Hh) signaling
Chemical Name:	(2'R,3S,3'R,3'aS,6'S,6aS,6bS,7'aR,11aS,11bR)-1,2,3,3'a,4,4',5',6,6',6a,6b,7,7',7'a,8,11,11a,11b-Octadecahydro-3',6',10,11b-tetramethylspiro[9H-benzo[a]fluorene-9,2'(3'H)-furo[3,2-b]pyridin]-3-ol
Purity:	≥97% (HPLC)
Molecular Weight:	411.63

View Full Technical Details for Cyclopamine

Technical Data
Citations / Reviews (1)

Biological Activity for Cyclopamine

Cyclopamine is an inhibitor of hedgehog (Hh) signaling, likely via direct inhibition of Smoothened, the accessory protein to the putative Hh receptor Patched. Anticancer and teratogenic in vivo. Depletes stem-like cancer cells in glioblastoma and blocks tumor engraftment. Can also be used to induce differentiation of human embryonic stem cells (hESCs) into hormone-expressing endocrine cells.

Technical Data for Cyclopamine

M. Wt	411.63
Formula	C₂₇H₄₁NO₂
Storage	Desiccate at -20°C
Purity	≥97% (HPLC)
CAS Number	4449-51-8
PubChem ID	442972
InChi Key	QASFUMOKHFSJGL-LAFRSMQTSA-N
Smiles	O[C@H](C2)CC[C@@]1(C)C2=CC[C@]([C@@]([H])3CC[C@@]54O[C@@](C[C@H](C)CN6)([H])[C@]6([H])[C@H]5C)([H])[C@@]([H])1CC3=[C@@]4C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Cyclopamine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	ethanol	2.06	5

Preparing Stock Solutions for Cyclopamine

The following data is based on the product molecular weight 411.63.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.05 mM	48.59 mL	242.94 mL	485.87 mL
0.25 mM	9.72 mL	48.59 mL	97.17 mL
0.5 mM	4.86 mL	24.29 mL	48.59 mL
2.5 mM	0.97 mL	4.86 mL	9.72 mL

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References for Cyclopamine

References are publications that support the biological activity of the product.

D'Amour Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat.Biotechnol. 2006 PMID: 17053790
Scott Cancer: a twist in a hedgehog's tale. Nature 2003 PMID: 14574396
Bar Cyclopamine-mediated hedgehog pathway inhibition depletes stem-like cancer cells in glioblastoma. Stem Cells 2007 PMID: 17628016
Chen Inhibition of hedgehog signaling by direct binding of cyclopamine to smoothened. Genes Dev. 2002 PMID: 12414725