DIDS

Name: DIDS
Brand: Tocris Bioscience
SKU: 4523
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4523

(1)

Citations (28)

COA / SDS

Chloride channel blocker; TRPV1 modulator; RAD51 recombinase inhibitor

Key Product Details

Description:	Chloride channel blocker; TRPV1 modulator; RAD51 recombinase inhibitor
Chemical Name:	4,4'-Diisothiocyanato-2,2'-stilbenedisulfonic acid disodium salt
Purity:	≥95% (HPLC)
Molecular Weight:	498.48

View Full Technical Details for DIDS

Technical Data
Citations / Reviews (1)

Biological Activity for DIDS

DIDS is a ClC-Ka chloride channel blocker (IC₅₀ = 100 μM). Blocks the maxi chloride channel in apical membranes from human placenta. Displays antagonistic activity at TRPM4 and TRPC4 channels; potentiates agonist-induced TRPV1 currents (IC₅₀ = 4.88 μM in rat DRG neurons). Inhibits RAD51 recombinase activity (K_D = 2 μM).

Technical Data for DIDS

M. Wt	498.48
Formula	C₁₆H₈N₂Na₂O₆S₄
Storage	Store at +4°C
Purity	≥95% (HPLC)
CAS Number	67483-13-0
PubChem ID	5702690
InChi Key	GEPAYBXVXXBSKP-SEPHDYHBSA-L
Smiles	[Na+].[Na+].[O-]S(=O)(=O)C1=C(\C=C\C2=C(C=C(C=C2)N=C=S)S([O-])(=O)=O)C=CC(=C1)N=C=S

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DIDS

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	Potassium bicarbonate (0.1M)	2.49	5

Preparing Stock Solutions for DIDS

The following data is based on the product molecular weight 498.48.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.05 mM	40.12 mL	200.61 mL	401.22 mL
0.25 mM	8.02 mL	40.12 mL	80.24 mL
0.5 mM	4.01 mL	20.06 mL	40.12 mL
2.5 mM	0.80 mL	4.01 mL	8.02 mL

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Reconstitution Calculator

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References for DIDS

References are publications that support the biological activity of the product.

Riquelme and Parra Regulation of human placental chloride channel by arachidonic acid and other cis unsaturated fatty acids. Am.J.Obstet.Gynecol. 1999 PMID: 9988821
Wulff New light on the "old" chloride channel blocker DIDS. ACS Chem.Biol. 2008 PMID: 18642798
Zhang Agonist-dependent potentiation of vanilloid receptor transient receptor potential vanilloid type 1 function by stilbene derivatives. Mol.Pharmacol. 2012 PMID: 22328719
Ishida DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange. Nucleic Acids Res. 2009 PMID: 19336413