DMNPE-4 AM-caged-calcium

Name: DMNPE-4 AM-caged-calcium
Brand: Tocris Bioscience
SKU: 5948

Tocris Bioscience, part of Bio-Techne | Catalog # 5948

Citations (2)

COA / SDS

Calcium cage; cell permeable

Key Product Details

Description:	Calcium cage; cell permeable
Chemical Name:	Bis(acetoxymethyl) 3,12-bis(2-(acetoxymethoxy)-2-oxoethyl)-5-(4,5-dimethoxy-2-nitrophenyl)-6,9-dioxa-3,12-diazatetradecane-1,14-dioate
Purity:	≥95% (HPLC)
Molecular Weight:	849.74

View Full Technical Details for DMNPE-4 AM-caged-calcium

Technical Data
Citations

Biological Activity for DMNPE-4 AM-caged-calcium

DMNPE-4 AM-caged-calcium is a calcium cage. In the presence of calcium, the compound will chelate and cage calcium (K_d values are 48 and 19 nM at pH 7.2 and 7.4, respectively; K_d after uncaging = 2 mM). DMNPE-4 AM-caged-calcium is selective for Ca²⁺ over Mg²⁺ (K_d = 10 mM). Rapid and efficient calcium release occurs upon photolysis at 350 nm. It can also be used for two-photon uncaging. Extinction coefficient of 5120 M^-1 cm^-1, quantum yield 0.09. Cell permeable.

Technical Data for DMNPE-4 AM-caged-calcium

M. Wt	849.74
Formula	C₃₄H₄₇N₃O₂₂
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	2253744-58-8
PubChem ID	137919855
InChi Key	UUZFENOXYOBVDU-UHFFFAOYSA-N
Smiles	O=C(OCOC(C)=O)CN(CC(OCOC(C)=O)=O)CCOCCOC(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(C=C1OC)=C(C=C1OC)[N+]([O-])=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DMNPE-4 AM-caged-calcium

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	84.97	100

Preparing Stock Solutions for DMNPE-4 AM-caged-calcium

The following data is based on the product molecular weight 849.74.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.18 mL	5.88 mL	11.77 mL
5 mM	0.24 mL	1.18 mL	2.35 mL
10 mM	0.12 mL	0.59 mL	1.18 mL
50 mM	0.02 mL	0.12 mL	0.24 mL

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References for DMNPE-4 AM-caged-calcium

References are publications that support the biological activity of the product.

Gordon Brain metabolism dictates the polarity of astrocyte control over arterioles. Nature 2008 PMID: 18971930
Ellis-Davies Tuning caged calcium: photolabile analogues of EGTA with improved optical and chelation properties. Cell Calcium 2006 PMID: 16303177
Wang Spontaneous activity of cochlear hair cells triggered by fluid secretion mechanism in adjacent support cells Cell 2015 PMID: 26627734