DOPE

Name: DOPE
Brand: Tocris Bioscience
SKU: 7175

Tocris Bioscience, part of Bio-Techne | Catalog # 7175

Citations (2)

COA / SDS

Liposome/lipoplex transfection enhancer

Key Product Details

Description:	Liposome/lipoplex transfection enhancer
Chemical Name:	(9Z)-1,1'-[(1R)-1-[[[(2-Aminoethoxy)hydroxyphosphinyl]oxy]methyl]-1,2-ethanediyl] 9-octadecenoic acid ester
Purity:	≥95% (HPLC)
Molecular Weight:	744.05

View Full Technical Details for DOPE

Technical Data
Citations

Biological Activity for DOPE

DOPE is a liposome/lipoplex transfection reagent. Enhances liposome and lipoplex transfection efficiency in combination with DA, DOTAP or DC-cholesterol (Cat. No. 7176).

Please note: exact ratio for use and transfection efficiency is dependent on cell line used.

Technical Data for DOPE

M. Wt	744.05
Formula	C₄₁H₇₈NO₈P
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	4004-05-1
PubChem ID	9546757
InChi Key	MWRBNPKJOOWZPW-NYVOMTAGSA-N
Smiles	CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@H](CO[P](O)(=O)OCCN)OC(=O)CCCCCCC\C=C/CCCCCCCC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DOPE

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	chloroform	74.4	100

Preparing Stock Solutions for DOPE

The following data is based on the product molecular weight 744.05.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.34 mL	6.72 mL	13.44 mL
5 mM	0.27 mL	1.34 mL	2.69 mL
10 mM	0.13 mL	0.67 mL	1.34 mL
50 mM	0.03 mL	0.13 mL	0.27 mL

Molarity Calculator

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Reconstitution Calculator

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References for DOPE

References are publications that support the biological activity of the product.

Kim DOTAP/DOPE ratio and cell type determine transfection efficiency with DOTAP-liposomes. Biochim.Biophys.Acta. 2015 PMID: 26112463
Mochizuki The role of the helper lipid dioleoylphosphatidylethanolamine (DOPE) for DNA transfection cooperating with a cationic lipid bearing ethylenediamine. Biochim.Biophys.Acta. 2013 PMID: 23092705