DOTAP

Name: DOTAP
Brand: Tocris Bioscience
SKU: 7179

Tocris Bioscience, part of Bio-Techne | Catalog # 7179

COA / SDS

Cationic agent for liposome and LNP formation

Key Product Details

Description:	Cationic agent for liposome and LNP formation
Alternative Names:	1,2-dioleoyl-3-trimethylammoniumpropane
Chemical Name:	N,N,N-Trimethyl-2,3-bis(oleoyloxy)propan-1-aminium chloride
Purity:	≥95% (HPLC)
Molecular Weight:	698.54

View Full Technical Details for DOTAP

Technical Data
Citations

Biological Activity for DOTAP

DOTAP is a cationic agent used in liposome formation. Facilitates the transfection of DNA, RNA, and other molecules with negative charges into eukaryotic cells. Can be used to generate selective organ-targeting (SORT) lipid nanoparticles.

For more information on how to prepare MC3 lipid nanoparticles for RNA delivery, please see our protocol.

Technical Data for DOTAP

M. Wt	698.54
Formula	C₄₂H₈₀ClNO₄
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	132172-61-3
InChi Key	KSXTUUUQYQYKCR-LQDDAWAPNA-M
Smiles	C(OC(CCCCCCC/C=C\CCCCCCCC)=O)(COC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DOTAP

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	69.85	100
	chloroform	69.85	100

Preparing Stock Solutions for DOTAP

The following data is based on the product molecular weight 698.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.43 mL	7.16 mL	14.32 mL
5 mM	0.29 mL	1.43 mL	2.86 mL
10 mM	0.14 mL	0.72 mL	1.43 mL
50 mM	0.03 mL	0.14 mL	0.29 mL

Molarity Calculator

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Reconstitution Calculator

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References for DOTAP

References are publications that support the biological activity of the product.

Wang Preparation of selective organ-targeting (SORT) lipid nanoparticles (LNPs) using multiple technical methods for tissue-specific mRNA delivery. Nat.Protoc. 2023 PMID: 36316378