EM 163

Name: EM 163
Brand: Tocris Bioscience
SKU: 7010

Tocris Bioscience, part of Bio-Techne | Catalog # 7010

COA / SDS

TIR-TIR interaction inhibitor

Key Product Details

Description:	TIR-TIR interaction inhibitor
Chemical Name:	1-Methyl-2,5-bis[[[(1S)-2-methyl-1-(1-pyrrolidinylcarbonyl)propyl](3-phenylpropyl)amino]carbonyl]pyridinium iodide
Purity:	≥98% (HPLC)
Molecular Weight:	849.9

View Full Technical Details for EM 163

Technical Data
Citations

Biological Activity for EM 163

EM 163 is a TIR-TIR interaction inhibitor, that is a TIR (Toll/ interleukin-1 receptor) domain peptidomimetic of MyD88 protein. EM 163 targets TIR domains in IL-1 receptors blocking the interaction with MyD88. EM 163 inhibits staphylococcal enterotoxin B (SEB)-induced inflammatory cytokine production in vivo. EM 163 protects mice from SEB shock-induced death. In rat hippocampal neurons in vitro, EM 163 blocks the activation of p38 and the inhibitory effects of IL-1β on protein synthesis triggered by chemically-induced long-term potentiation (LTP).

Technical Data for EM 163

M. Wt	849.9
Formula	C₄₄H₆₀IN₅O₄
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1206480-93-4
PubChem ID	44602511
InChi Key	WJCJDZIXEVJIKZ-OFUZFLEOSA-M
Smiles	CC([C@@H](C(N1CCCC1)=O)N(C(C2=C[N+](C)=C(C(N([C@H](C(N3CCCC3)=O)C(C)C)CCCC4=CC=CC=C4)=O)C=C2)=O)CCCC5=CC=CC=C5)C.[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for EM 163

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	84.99	100

Preparing Stock Solutions for EM 163

The following data is based on the product molecular weight 849.9.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.18 mL	5.88 mL	11.77 mL
5 mM	0.24 mL	1.18 mL	2.35 mL
10 mM	0.12 mL	0.59 mL	1.18 mL
50 mM	0.02 mL	0.12 mL	0.24 mL

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References for EM 163

References are publications that support the biological activity of the product.

Kissner Therapeutic inhibition of pro-inflammatory signaling and toxicity to staphylococcal enterotoxin B by a synthetic dimeric BB-loop mimetic of MyD88. PLoS One 2012 PMID: 22848400
Lucas and Maes Role of the toll like receptor (TLR) radical cycle in chronic inflammation: possible treatments targeting the TLR4 pathway. Mol.Neurobiol. 2013 PMID: 23436141
Prieto Inhibition of LTP-induced translation by IL-1β reduces the level of newly synthesized proteins in hippocampal dendrites. ACS Chem.Neurosci. 2019 PMID: 30695637