Go 6976

Name: Go 6976
Brand: Tocris Bioscience
SKU: 2253

Tocris Bioscience, part of Bio-Techne | Catalog # 2253

Citations (67)

COA / SDS

Potent protein kinase C inhibitor; selective for α and β isozymes

Key Product Details

Description:	Potent protein kinase C inhibitor; selective for α and β isozymes
Chemical Name:	5,6,7,13-Tetrahydro-13-methyl-5-oxo-12H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-12-propanenitrile
Purity:	≥95% (HPLC)
Molecular Weight:	378.43

View Full Technical Details for Go 6976

Technical Data
Citations

Biological Activity for Go 6976

Go 6976 is a potent protein kinase C (PKC) inhibitor (IC₅₀ = 7.9 nM). Discriminates between Ca²⁺-dependent and -independent isoforms of PKC in vitro; selectively inhibits PKCα and PKCβ1 (IC₅₀ values are 2.3 and 6.2 nM respectively). Does not inhibit the activity of PKCδ, -ε, or -ζ (IC₅₀ > 3μM). Also inhibits TrkA, TrkB, JAK2 and JAK3 tyrosine kinases (IC₅₀ values are 5, 30, 130 and 370 nM respectively), PKCμ (PKD1) and the mitotic spindle checkpoint.

Technical Data for Go 6976

M. Wt	378.43
Formula	C₂₄H₁₈N₄O
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	136194-77-9
PubChem ID	3501
InChi Key	VWVYILCFSYNJHF-UHFFFAOYSA-N
Smiles	O=C(NC3)C1=C3C(C4=CC=CC=C4N5CCC#N)=C5C2=C1C6=C(C=CC=C6)N2C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Go 6976

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	3.78	10

Preparing Stock Solutions for Go 6976

The following data is based on the product molecular weight 378.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	26.42 mL	132.12 mL	264.25 mL
0.5 mM	5.28 mL	26.42 mL	52.85 mL
1 mM	2.64 mL	13.21 mL	26.42 mL
5 mM	0.53 mL	2.64 mL	5.28 mL

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References for Go 6976

References are publications that support the biological activity of the product.

Stolz Pharmacologic abrogation of the mitotic spindle checkpoint by an indolocarbazole discovered by cellular screening efficiently kills cancer cells. Cancer Res. 2009 PMID: 19366805
Grandage Go6976 is a potent inhibitor of JAK2 and FLT3 tyrosine kinases with significant activity in primary acute myeloid leukaemia cells. Br.J.Haematol. 2006 PMID: 16956345
Rennecke Immunological demonstration of protein kinase Cμ in murine tissues and various cell lines. Eur.J.Biochem. 1996 PMID: 8973662
Behrens Go 6976 is a potent inhibitor of neurotrophin-receptor intrinsic tyrosine kinase. J.Neurochem. 1999 PMID: 10037462
Martiny-Baron Selective inhibition of protein kinase C isozymes by the indolocarbazole Gö 6976 J.Biol.Chem. 1993 PMID: 8486620