GW 280264X

Name: GW 280264X
Brand: Tocris Bioscience
SKU: 7030

Tocris Bioscience, part of Bio-Techne | Catalog # 7030

Citations (4)

COA / SDS

Potent ADAM10 and ADAM17 inhibitor

Key Product Details

Description:	Potent ADAM10 and ADAM17 inhibitor
Chemical Name:	Phenylmethyl N-[(5S)-5-[[(2R,3S)-3-(formylhydroxyamino)-2-(2-methylpropyl)-1-oxohexyl]amino]-6-oxo-6-(2-thiazolylamino)hexyl]carbamate
Purity:	≥98% (HPLC)
Molecular Weight:	575.73

View Full Technical Details for GW 280264X

Technical Data
Citations

Biological Activity for GW 280264X

GW 280264X is a potent ADAM (A Disintegrin and Metalloproteinase) 17 and ADAM10 inhibitor (IC₅₀ values of 8 nM and 11.5 nM, respectively). It inhibits constitutive and PMA-inducible CX3CL1 shedding and increases adhesive properties in CX3CL1 transfected cells (IC₅₀ = 1 μM in COS-7 and ECV-304 cells). GW 280264X treatment in mice significantly improves functional recovery after spinal cord injury.

Compound Libraries for GW 280264X

GW 280264X is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for GW 280264X

M. Wt	575.73
Formula	C₂₈H₄₁N₅O₆S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	866924-39-2
PubChem ID	91885425
InChi Key	SKJLITFHSZNZMQ-SGNDLWITSA-N
Smiles	CCC[C@H](N(C=O)O)[C@H](C(N[C@H](C(NC1=NC=CS1)=O)CCCCNC(OCC2=CC=CC=C2)=O)=O)CC(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for GW 280264X

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	57.57	100

Preparing Stock Solutions for GW 280264X

The following data is based on the product molecular weight 575.73.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.74 mL	8.68 mL	17.37 mL
5 mM	0.35 mL	1.74 mL	3.47 mL
10 mM	0.17 mL	0.87 mL	1.74 mL
50 mM	0.03 mL	0.17 mL	0.35 mL

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References for GW 280264X

References are publications that support the biological activity of the product.

Sommer ADAM17-deficiency on microglia but not on macrophages promotes phagocytosis and functional recovery after spinal cord injury Brain Behav.Immun. 2019 PMID: 30851378
Hundhausen The disintegrin-like metalloproteinase ADAM10 is involved in constitutive cleavage of CX3CL1 (fractalkine) and regulates CX3CL1-mediated cell-cell adhesion. Blood 2003 PMID: 12714508