Hydroxychloroquine sulfate

Name: Hydroxychloroquine sulfate
Brand: Tocris Bioscience
SKU: 5648
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5648

(1)

Citations (5)

COA / SDS

Autophagy inhibitor; also TLR9 inhibitor

Key Product Details

Description:	Autophagy inhibitor; also TLR9 inhibitor
Chemical Name:	2-[[4-[(7-Chloro-4-quinolinyl)amino]pentyl]ethylamino]ethanol sulfate
Purity:	≥99% (HPLC)
Molecular Weight:	433.95

View Full Technical Details for Hydroxychloroquine sulfate

Technical Data
Citations / Reviews (1)

Biological Activity for Hydroxychloroquine sulfate

Hydroxychloroquine sulfate is an autophagy inhibitor. Also inhibits TLR9. Inhibits growth and induces apoptosis of renal cancer cells in vitro. Additionally inhibits PRC2 by inhibiting the allosteric binding of PRC2 to EED (embryonic ectoderm development) within the H3K27me3-binding pocket, and reduces H3K27me3 levels in multiple myeloma cells in vitro. Inhibits SARS-CoV-2 viral infection, in vitro (EC₅₀ values in μM range and dependent on viral RNA copy number). Hydroxychloroquine improves efficacy of adeno-associated viral gene transduction in vivo and in vitro. Also antimalarial and immunomodulator.

Tocris products are for biomedical research use only. They are not intended for human or veterinary use.

Technical Data for Hydroxychloroquine sulfate

M. Wt	433.95
Formula	C₁₈H₂₈ClN₃O₅S
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	747-36-4
PubChem ID	12947
InChi Key	JCBIVZZPXRZKTI-UHFFFAOYSA-N
Smiles	ClC(C=C1)=CC2=C1C(NC(CCCN(CC)CCO)C)=CC=N2.OS(=O)(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Hydroxychloroquine sulfate

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	43.4	100

Preparing Stock Solutions for Hydroxychloroquine sulfate

The following data is based on the product molecular weight 433.95.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.30 mL	11.52 mL	23.04 mL
5 mM	0.46 mL	2.30 mL	4.61 mL
10 mM	0.23 mL	1.15 mL	2.30 mL
50 mM	0.05 mL	0.23 mL	0.46 mL

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References for Hydroxychloroquine sulfate

References are publications that support the biological activity of the product.

Chandler Enhancement of adeno-associated virus-mediated gene therapy using hydroxychloroquine in murine and human tissues. Mol.Ther.Methods Clin.Dev. 2019 PMID: 31309129
Liu Hydroxychloroquine, a less toxic derivative of chloroquine, is effective in inhibiting SARS-CoV-2 infection in vitro. Cell Discovery 2020 PMID: 32194981
Lamphier Novel small molecule inhibitors of TLR7 and TLR9: mechanism of action and efficacy in vivo. Mol.Pharmacol. 2014 PMID: 24342772
Catalano A drug repurposing screening reveals a novel epigenetic activity of hydroxychloroquine. Eur.J.Med.Chem. 2019 PMID: 31550663
Lee Hydroxychloroquine Destabilizes Phospho-S6 in Human Renal Carcinoma Cells. PLoS ONE 2015 PMID: 26134285