Hygromycin B

Name: Hygromycin B
Brand: Tocris Bioscience
SKU: 4137
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4137

(1)

Citations (4)

COA / SDS

Aminoglycoside antibiotic; active against prokaryotic and eukaryotic cells

Key Product Details

Description:	Aminoglycoside antibiotic; active against prokaryotic and eukaryotic cells
Chemical Name:	O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
Purity:	≥90% (HPLC)
Molecular Weight:	527.52

View Full Technical Details for Hygromycin B

Technical Data
Citations / Reviews (1)

Biological Activity for Hygromycin B

Hygromycin B is an aminoglycoside antibiotic. Active against both prokaryotic and eukaryotic cells. Inhibits protein synthesis by binding to the 30S subunit and inhibiting the ribosomal translocation step of elongation.

Technical Data for Hygromycin B

M. Wt	527.52
Formula	C₂₀H₃₇N₃O₁₃
Storage	Store at +4°C
Purity	≥90% (HPLC)
CAS Number	31282-04-9
PubChem ID	90488914
InChi Key	GRRNUXAQVGOGFE-SZNRJEGWSA-N
Smiles	O[C@H]([C@@H](NC)C[C@@H](N)[C@@H]4O)C4O[C@H]1[C@]2([H])[C@](OC3(O[C@]([H])([C@@H](N)CO)[C@H](O)[C@@H](O)[C@H]3O)O2)([H])[C@@H](O)[C@@H](CO)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Hygromycin B

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	50

Preparing Stock Solutions for Hygromycin B

The following data is based on the product molecular weight 527.52.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.95 mM	2.00 mL	9.98 mL	19.95 mL
4.75 mM	0.40 mL	2.00 mL	3.99 mL
9.5 mM	0.20 mL	1.00 mL	2.00 mL
47.5 mM	0.04 mL	0.20 mL	0.40 mL

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Reconstitution Calculator

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References for Hygromycin B

References are publications that support the biological activity of the product.

Brodersen The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell 2000 PMID: 11163189
Ganoza and Kiel A ribosomal ATPase is a target for hygromycin B inhibition on Escherichia coli ribosomes. Antimicrob.Agents Chemother. 2001 PMID: 11557474
Blochlinger and Diggelmann Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eukaryotic cells. Mol.Cell.Biol. 1984 PMID: 6098829