Indisulam

Name: Indisulam
Brand: Tocris Bioscience
SKU: 6782

Tocris Bioscience, part of Bio-Techne | Catalog # 6782

COA / SDS

Molecular glue; pre-mRNA splicing modulator

Key Product Details

Description:	Molecular glue; pre-mRNA splicing modulator
Chemical Name:	N¹-(3-Chloro-1H-indol-7-yl)-1,4-benzenedisulfonamide
Purity:	≥98% (HPLC)
Molecular Weight:	385.84

View Full Technical Details for Indisulam

Technical Data
Citations

Biological Activity for Indisulam

Indisulam is an acts as a molecular glue to induce proteosomal degradation of mRNA splicing factor RBM39 (also designated CAPERα, HCC1, FSAP59, and RNPC2), via binding to DCAF15. Acts as a pre-mRNA splicing modulator (SPLAMs; splicing inhibitor sulfonamides), causes aberrant pre-mRNA splicing. Suppresses proliferation of cancer cell lines. Reduces viability of HCT-116 cells (IC₅₀ = 0.56 μM). Induces cell cycle arrest in the G₁ phase in cancer cell lines. Also a high affinity carbonic anhydrase isozyme XII (hCA XII) inhibitor (K_i = 3.0-5.7 nM).

Technical Data for Indisulam

M. Wt	385.84
Formula	C₁₄H₁₂ClN₃O₄S₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	165668-41-7
PubChem ID	216468
InChi Key	SETFNECMODOHTO-UHFFFAOYSA-N
Smiles	NS(=O)(C1=CC=C(S(=O)(NC2=CC=CC3=C2NC=C3Cl)=O)C=C1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Indisulam

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	38.58	100

Preparing Stock Solutions for Indisulam

The following data is based on the product molecular weight 385.84.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.59 mL	12.96 mL	25.92 mL
5 mM	0.52 mL	2.59 mL	5.18 mL
10 mM	0.26 mL	1.30 mL	2.59 mL
50 mM	0.05 mL	0.26 mL	0.52 mL

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References for Indisulam

References are publications that support the biological activity of the product.

Vullo Carbonic anhydrase inhibitors. Inhibition of the transmembrane isozyme XII with sulfonamides-a new target for the design of antitumor and antiglaucoma drugs? Bioorg.Med.Chem.Lett. 2005 PMID: 15686894
Di Function, clinical application, and strategies of Pre-mRNA splicing in cancer. Cell Death Differ. 2018 PMID: 30464224
Owa Discovery of novel antitumor sulfonamides targeting G₁ phase of the cell cycle. J.Med.Chem. 1999 PMID: 10508428
Ozawa E7070, a novel sulphonamide agent with potent antitumour activity in vitro and in vivo. Eur.J.Cancer. 2001 PMID: 11677118
Uehara Selective degradation of splicing factor CAPERa by anticancer sulfonamides. Nat.Chem.Biol. 2017 PMID: 28437394
Han Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. Science 2017 PMID: 28302793