Ispinesib

Name: Ispinesib
Brand: Tocris Bioscience
SKU: 5408

Tocris Bioscience, part of Bio-Techne | Catalog # 5408

Citations (4)

COA / SDS

High affinity and selective allosteric KSP inhibitor

Key Product Details

Description:	High affinity and selective allosteric KSP inhibitor
Alternative Names:	SB 715992
Chemical Name:	N-(3-Aminopropyl)-N-[(1R)-1-[7-chloro-3,4-dihydro-4-oxo-3-(phenylmethyl)-2-quinazolinyl]-2-methylpropyl]-4-methylbenzamide
Purity:	≥98% (HPLC)
Molecular Weight:	517.06

View Full Technical Details for Ispinesib

Technical Data
Citations

Biological Activity for Ispinesib

Ispinesib is a high affinity allosteric kinesin spindle protein (KSP) inhibitor (K_{i app} = 1.7 nM). Exhibits >10,000-fold selectivity for KSP over a range of other mitotic kinesins. Inhibits proliferation and induces apoptosis of prostate and breast cancer cells in vitro. Induces tumor regression of breast cancer cell xenografts in mice.

Compound Libraries for Ispinesib

Ispinesib is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Ispinesib

M. Wt	517.06
Formula	C₃₀H₃₃ClN₄O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	336113-53-2
PubChem ID	6851740
InChi Key	QJZRFPJCWMNVAV-HHHXNRCGSA-N
Smiles	O=C2N(CC3=CC=CC=C3)C([C@@H]([C@@H](C)C)N(CCCN)C(C4=CC=C(C)C=C4)=O)=NC1=CC(Cl)=CC=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Ispinesib

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	51.71	100
	ethanol	51.71	100

Preparing Stock Solutions for Ispinesib

The following data is based on the product molecular weight 517.06.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.93 mL	9.67 mL	19.34 mL
5 mM	0.39 mL	1.93 mL	3.87 mL
10 mM	0.19 mL	0.97 mL	1.93 mL
50 mM	0.04 mL	0.19 mL	0.39 mL

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References for Ispinesib

References are publications that support the biological activity of the product.

Davis Increased therapeutic potential of an experimental anti-mitotic inhibitor SB715992 by genistein in PC-3 human prostate cancer cell line. BMC Cancer 2006 PMID: 16433906
Purcell Activity of the kinesin spindle protein inhibitor ispinesib (SB-715992) in models of breast cancer. Clin.Cancer Res. 2010 PMID: 20068098
Lad Mechanism of inhibition of human KSP by ispinesib. Biochemistry 2008 PMID: 18290633