ISX 9

Name: ISX 9
Brand: Tocris Bioscience
SKU: 4439

Tocris Bioscience, part of Bio-Techne | Catalog # 4439

Citations (17)

COA / SDS

Neurogenic agent; induces neuronal differentiation of SVZ progenitors and also induces cardiomyogenic differentiation

Key Product Details

Chemical Name:	N-Cyclopropyl-5-(2-thienyl)-3-isoxazolecarboxamide
Purity:	≥98%

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Technical Data
Citations

Biological Activity for ISX 9

ISX 9 is a neurogenic agent. Mediates neuroD reporter gene induction via activation of Ca²⁺ influx; increases expression of neurogenic differentiation 1 (NeuroD1) transcription factor. Induces neuronal differentiation in human cortical neuronal cells (HCN), adult mouse whole brain (MWB) and subventricular zone (SVZ) progenitors. ISX 9 is also shown to induce cardiomyogenic differentiation of Notch-activated epicardium-derived cells in vitro. When used in combination with other small molecules, ISX 9 directs differentiation of pancreatic progenitors towards β cells.

ISX 9 synthesized to Ancillary Material Grade also available.

For more information about how ISX 9 may be used, see our protocol: Transdifferentiating Fibroblasts into Neurons.

Technical Data for ISX 9

Formula	C₁₁H₁₀N₂O₂S
Storage	Store at +4°C
Purity	≥98%
CAS Number	832115-62-5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ISX 9

Solubility

Soluble Soluble to 100 mM in DMSO and to 50 mM in ethanol

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References for ISX 9

References are publications that support the biological activity of the product.

A Tsakmaki, P Fonseca Pe, P Pavlidis, B Hayee, GA Bewick ISX-9 manipulates endocrine progenitor fate revealing conserved intestinal lineages in mouse and human organoids Mol Metab, 2020;34(0):157-173. 2020 PMID: 32180555
Russell et al. Targeting native adult heart progenitors with cardiogenic small molecules. ACS Chem.Biol., 2012;7:1067 PMID: 22413910
Schneider et al. Small-molecule activation of neuronal cell fate. Nat.Chem.Biol, 2008;4:408 PMID: 18552832
Zhang et al. Small-molecule blocks malignant astrocyte proliferation and induces neuronal gene expression. Differentiation, 2011;81:233 PMID: 21419563
Dioum et al. A small molecule differentiation inducer increases Ins production by pancreatic β cells. Proc.Natl.Acad.Sci.U.S.A., 2011;108:20713 PMID: 22143803

ISX 9

Tocris Bioscience, part of Bio-Techne | Catalog # 4439

Key Product Details

Biological Activity for ISX 9

Technical Data for ISX 9

Solubility for ISX 9

Dilution Calculator

Reconstitution Calculator

References for ISX 9

Product Documents for ISX 9

COA

Certificate of Analysis

SDS

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