JH-XI-10-02

Name: JH-XI-10-02
Brand: Tocris Bioscience
SKU: 7304

Tocris Bioscience, part of Bio-Techne | Catalog # 7304

COA / SDS

CDK8 Degrader (PROTAC®)

Key Product Details

Description:	CDK8 Degrader (PROTAC^®)
Chemical Name:	15-[[2-(2,6-Dioxo-3-piperidinyl)-2,3-dihydro-1,3-dioxo-1H-isoindol-4-yl]amino]-N-[(3β,5α,17β)-17-(7-isoquinolinyl)androstan-3-yl]-N-methyl-4,7,10,13-tetraoxapentadecanamide
Purity:	≥98% (HPLC)
Molecular Weight:	920.16

View Full Technical Details for JH-XI-10-02

Technical Data
Citations

Biological Activity for JH-XI-10-02

JH-XI-10-02 is a CDK8 Degrader (PROTAC^®) comprising the cereblon binding ligand Pomalidomide (Cat. No. 6302) joined by a linker to a CDK8 inhibitor. JH-XI-10-02 (1 μM) induces significant proteasome-dependent degradation of CDK8 in Jurkat cells after 24 h, with no significant effect on CDK19 levels.

CDK8 antibody validated for Western Blot also available: Catalog # NBP2-92972.

Licensing Information

Sold under license from Dana-Farber Cancer Institute.

Technical Data for JH-XI-10-02

M. Wt	920.16
Formula	C₅₃H₆₉N₅O₉
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2209085-22-1
PubChem ID	145708490
InChi Key	JECHBTRAPARMGI-GFTKVEOVSA-N
Smiles	[H][C@@]12CC[C@@H]([C@]1(CC[C@]3([C@]2(CC[C@]4(C[C@@H](N(C(CCOCCOCCOCCOCCNC5=C6C(N(C(C6=CC=C5)=O)C7CCC(NC7=O)=O)=O)=O)C)CC[C@]34C)[H])[H])[H])C)C8=CC9=C(C=C8)C=CN=C9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for JH-XI-10-02

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	46.01	50

Preparing Stock Solutions for JH-XI-10-02

The following data is based on the product molecular weight 920.16.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	2.17 mL	10.87 mL	21.74 mL
2.5 mM	0.43 mL	2.17 mL	4.35 mL
5 mM	0.22 mL	1.09 mL	2.17 mL
25 mM	0.04 mL	0.22 mL	0.43 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for JH-XI-10-02

References are publications that support the biological activity of the product.

Hatcher Development of highly potent and selective steroidal inhibitors and degraders of CDK8. ACS Med.Chem.Lett. 2018 PMID: 29937979