JHU 37160

Name: JHU 37160
Brand: Tocris Bioscience
SKU: 7198

Tocris Bioscience, part of Bio-Techne | Catalog # 7198

COA / SDS

High affinity and highly potent hM3Dq and hM4Di DREADD agonist; blood brain barrier penetrant

Key Product Details

Description:	High affinity and highly potent hM₃D_q and hM₄D_i DREADD agonist; blood brain barrier penetrant
Chemical Name:	8-Chloro-11-(4-ethylpiperazin-1-yl)-4-fluoro-5H-dibenzo[b,e][1,4]diazepine
Purity:	≥98% (HPLC)
Molecular Weight:	358.85

View Full Technical Details for JHU 37160

Technical Data
Citations

Biological Activity for JHU 37160

JHU 37160 is a high affinity and highly potent activator of hM₃D_q and hM₄D_i DREADDs (K_i values are 1.9 nM and 3.6 nM for hM₃D_q and hM₄D_i in vitro, respectively; EC₅₀ values are 0.2 nM and 18.5 nM for hM₄D_i and hM₃D_q in vitro, respectively). Displays approximately 25-fold higher affinity for hM₄D_i compared to DREADD agonist 21 (Cat. No. 5548). Selectively displaces [³H]clozapine from DREADDs in vivo, but not from other clozapine binding sites. Inhibits locomotor activity in mice expressing hM₃D_q and hM₄D_i in D₁-expressing neurons, and increases hM₃D_q-stimulated locomotion in rats expressing hM₃D_q in TH-expressing neurons. Brain penetrant in mice, rats and non-human primates.

Compound Libraries for JHU 37160

JHU 37160 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for JHU 37160

M. Wt	358.85
Formula	C₁₉H₂₀ClFN₄
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	2369979-68-8
PubChem ID	139033723
InChi Key	SWSCWOSASZXIRK-UHFFFAOYSA-N
Smiles	CCN1CCN(C2=NC3=CC(Cl)=CC=C3NC4=C(C=CC=C24)F)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for JHU 37160

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	17.94	50

Preparing Stock Solutions for JHU 37160

The following data is based on the product molecular weight 358.85.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	5.57 mL	27.87 mL	55.73 mL
2.5 mM	1.11 mL	5.57 mL	11.15 mL
5 mM	0.56 mL	2.79 mL	5.57 mL
25 mM	0.11 mL	0.56 mL	1.11 mL

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References for JHU 37160

References are publications that support the biological activity of the product.

Bonaventura High-potency ligands for DREADD imaging and activation in rodents and monkeys. Nat.Commun. 2019 PMID: 31604917