L-655,708

Name: L-655,708
Brand: Tocris Bioscience
SKU: 1327

Tocris Bioscience, part of Bio-Techne | Catalog # 1327

Citations (41)

COA / SDS

Benzodiazepine inverse agonist; selective for α5-containing GABAA receptors

Key Product Details

Description:	Benzodiazepine inverse agonist; selective for α5-containing GABA_A receptors
Chemical Name:	11,12,13,13a-Tetrahydro-7-methoxy-9-oxo-9H-imidazo[1,5-a]pyrrolo[2,1-c][1,4]benzodiazepine-1-carboxylic acid, ethyl ester
Purity:	≥98% (HPLC)
Molecular Weight:	341.37

View Full Technical Details for L-655,708

Technical Data
Citations

Biological Activity for L-655,708

L-655,708 is a potent, selective inverse agonist for the benzodiazepine site of GABA_A receptors containing the α5 subunit (K_i = 0.45 nM). Displays 50-100-fold selectivity over GABA_A receptors containing α1, α2, α3 or α6 subunits in combination with β3 and γ2. Enhances LTP in a mouse hippocampal slice model and increases spatial learning, without displaying proconvulsant activity.

Compound Libraries for L-655,708

L-655,708 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for L-655,708

M. Wt	341.37
Formula	C₁₈H₁₉N₃O₄
Storage	Desiccate at +4°C
Purity	≥98% (HPLC)
CAS Number	130477-52-0
PubChem ID	5311203
InChi Key	YKYOQIXTECBVBB-AWEZNQCLSA-N
Smiles	O=C(N3[C@]([H])2CCC3)C4=C(C=CC(OC)=C4)N1C2=C(C(OCC)=O)N=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for L-655,708

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	8.53	25

Preparing Stock Solutions for L-655,708

The following data is based on the product molecular weight 341.37.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.25 mM	11.72 mL	58.59 mL	117.17 mL
1.25 mM	2.34 mL	11.72 mL	23.43 mL
2.5 mM	1.17 mL	5.86 mL	11.72 mL
12.5 mM	0.23 mL	1.17 mL	2.34 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for L-655,708

References are publications that support the biological activity of the product.

Quirk [³H]L-655,708, a novel ligand selective for the benzodiazepine site of GABA_A receptors which contain the α5 subunit. Neuropharmacology 1996 PMID: 9014149
Sur Rat and human hippocampal α5 subunit-containing γ-aminobutyric acid_A receptors have α5α3γ2 pharmacological characteristics. Mol.Pharmacol. 1998 PMID: 9804628
Atack L-655,708 enhances cognition in rats but is not proconvulsant at a dose selective for α5-containing GABA_A receptors. Neuropharmacology 2006 PMID: 17046030