Lactacystin

Name: Lactacystin
Brand: Tocris Bioscience
SKU: 2267
Rating: 4 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 2267

(1)

Citations (35)

COA / SDS

Cell-permeable, potent and selective proteasome inhibitor

Key Product Details

Description:	Cell-permeable, potent and selective proteasome inhibitor
Chemical Name:	(2R,3S,4R)-3-Hydroxy-2-[(1S)-1-hydroxy-2-methylpropyl]-4-methyl-5-oxo-2-pyrrolidinecarboxy-N-acetyl-L-cysteine thioester
Purity:	≥95% (HPLC)
Molecular Weight:	376.42

View Full Technical Details for Lactacystin

Technical Data
Citations / Reviews (1)

Biological Activity for Lactacystin

Lactacystin is a cell-permeable, potent and selective proteasome inhibitor. A Streptomyces metabolite that is thought to bind irreversibly to the active site N-terminal threonine residue of the catalytic β-subunit of the 20S proteasome, thereby inhibiting its chymotrypsin and trypsin-like activities. Induces neurite outgrowth in Neuro 2a neuroblastoma cells and has been reported to induce apoptosis in human monoblast U937 cells. Also inhibits NF-κB activation (IC₅₀ = 10 μM).

Technical Data for Lactacystin

M. Wt	376.42
Formula	C₁₅H₂₄N₂O₇S
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	133343-34-7
PubChem ID	6610292
InChi Key	DAQAKHDKYAWHCG-RWTHQLGUSA-N
Smiles	O=C1N[C@]([C@@](SC[C@H](NC(C)=O)[C@](O)=O)=O)([C@H](C(C)C)O)[C@@H](O)[C@H]1C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Lactacystin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	3.76	10

Preparing Stock Solutions for Lactacystin

The following data is based on the product molecular weight 376.42.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	26.57 mL	132.83 mL	265.66 mL
0.5 mM	5.31 mL	26.57 mL	53.13 mL
1 mM	2.66 mL	13.28 mL	26.57 mL
5 mM	0.53 mL	2.66 mL	5.31 mL

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Reconstitution Calculator

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References for Lactacystin

References are publications that support the biological activity of the product.

Fenteany Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin. Science 1995 PMID: 7732382
Bellas Inhibition of NF-κB activity induces apoptosis in murine hepatocytes. Am.J.Pathol. 1997 PMID: 9327720
Omura Lactacystin, a novel microbial metabolite, induces neuritogenesis of neuroblastoma cells. J.Antibiot. 1991 PMID: 1848215