Methotrexate

Name: Methotrexate
Brand: Tocris Bioscience
SKU: 1230

Tocris Bioscience, part of Bio-Techne | Catalog # 1230

Citations (12)

COA / SDS

Cytotoxic agent

Key Product Details

Description:	Cytotoxic agent
Chemical Name:	N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid
Purity:	≥99% (HPLC)
Molecular Weight:	454.44

View Full Technical Details for Methotrexate

Technical Data
Citations

Biological Activity for Methotrexate

Methotrexate is a cytotoxic agent. Inhibits thymidylate synthetase and de novo purine synthesis. Potent folic acid antagonist; inhibits dihydrofolate reductase. Also inhibits Ras carboxyl methylation in DKOB8 cells, leading to decreased p44 and Akt activation.

Technical Data for Methotrexate

M. Wt	454.44
Formula	C₂₀H₂₂N₈O₅
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	59-05-2
PubChem ID	126941
InChi Key	FBOZXECLQNJBKD-ZDUSSCGKSA-N
Smiles	O=C(N[C@H]([C@](O)=O)CCC(O)=O)C(C=C3)=CC=C3N(CC2=NC1=C(N)N=C(N)N=C1N=C2)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Methotrexate

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO		100

Preparing Stock Solutions for Methotrexate

The following data is based on the product molecular weight 454.44.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.20 mL	11.00 mL	22.01 mL
5 mM	0.44 mL	2.20 mL	4.40 mL
10 mM	0.22 mL	1.10 mL	2.20 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

Molarity Calculator

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Reconstitution Calculator

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References for Methotrexate

References are publications that support the biological activity of the product.

Winter-Vann Targeting Ras signaling through inhibition of carboxyl methylation: an unexpected property of methotr. Proc.Natl.Acad.Sci.U.S.A. 2003 PMID: 12750467
Chu Mechanism of thymidylate synthase inhibition by methotr. in human neoplastic cell lines and normal human myeloid progenitor cells. J.Biol.Chem. 1990 PMID: 2341391
Jolivet The pharmacology and clinical use of methotr. N.Engl.J.Med. 1983 PMID: 6353235
Allegra Evidence for direct inhibition of de novo protein synthesis in human MCF-7 breast cells as a principal mode of metabolic inhibition by methotr. J.Biol.Chem. 1987 PMID: 2443493

Product Documents for Methotrexate

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.

SDS

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov