Methoxy-X04

Name: Methoxy-X04
Brand: Tocris Bioscience
SKU: 4920
Rating: 5 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4920

COA / SDS

Brain penetrant fluorescent amyloid β probe. Used for: detection and quantification of plaques, tangles and cerebrovascular amyloid. Application: confocal microscopy, suitable for in vivo imaging

Key Product Details

Description:	Brain penetrant fluorescent amyloid β probe. Used for: detection and quantification of plaques, tangles and cerebrovascular amyloid. Application: confocal microscopy, suitable for in vivo imaging
Chemical Name:	4,4'-[(2-methoxy-1,4-phenylene)di-(1E)-2,1-ethenediyl]bisphenol
Purity:	≥98% (HPLC)
Molecular Weight:	344.4

View Full Technical Details for Methoxy-X04

Technical Data
Citations / Reviews (2)

Biological Activity for Methoxy-X04

Key information: Methoxy-X04 is a blood-brain barrier penetrant fluorescent amyloid β (Aβ) probe. Suitable for in vivo imaging.

Used for: the detection and quantification of Aβ plaques, tangles and cerebrovascular amyloid. Gives high resolution fluorescent imaging on individual plaques in live animals by 2-photon microscopy. Methoxy-X04 co-localizes with CD68+ phagosomes in plaque-associated Iba1+ microglia; and it labels molecularly distinct plaque-associated microglia populations.

Application: confocal microscopy, in vivo imaging.

Properties and Photophysical Data: Methoxy-X04 displays high in vitro binding affinity (K_i = 26.8 nM); binds selectively to fibrillar β-sheet deposits. Excitation and emission maxima (λ) are 370 nm and 452 nm, respectively.

Optical Data for Methoxy-X04

λ_abs	370 nm
λ_em	452 nm

Technical Data for Methoxy-X04

M. Wt	344.4
Formula	C₂₃H₂₀O₃
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	863918-78-9
PubChem ID	16049314
InChi Key	FGYNZFHVGOFCMD-KHVHPYDTSA-N
Smiles	OC(C=C3)=CC=C3/C=C/C1=CC(OC)=C(/C=C/C2=CC=C(O)C=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Methoxy-X04

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	34.44	100
	ethanol	3.44	10

Preparing Stock Solutions for Methoxy-X04

The following data is based on the product molecular weight 344.4.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.90 mL	14.52 mL	29.04 mL
5 mM	0.58 mL	2.90 mL	5.81 mL
10 mM	0.29 mL	1.45 mL	2.90 mL
50 mM	0.06 mL	0.29 mL	0.58 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Methoxy-X04

References are publications that support the biological activity of the product.

Heneka NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice. Nature. 2013 PMID: 23254930
Grubman Transcriptional signature in microglia associated with Aβ plaque phagocytosis. Nat.Commun. 2021 PMID: 34021136
Yamanaka PPARγ/RXRα-induced and CD36-mediated microglial amyloid-β phagocytosis results in cognitive improvement in amyloid precursor protein/presenilin 1 mice. J.Neurosci. 2012 PMID: 23197723
Klunk Imaging Aβ plaques in living transgenic mice with multiphoton microscopy and methoxy-X04, a systemically administered Congo red derivative. J.Neuropathol.Exp.Neurol. 2002 PMID: 12230326
Bolmont Dynamics of the microglial/amyloid interaction indicate a role in plaque maintenance. J.Neurosci. 2008 PMID: 18417708