MG 132

Name: MG 132
Brand: Tocris Bioscience
SKU: 1748
Rating: 5 (4 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1748

(4)

Citations (340)

COA / SDS

Proteasome and calpain inhibitor. Inhibits NF-κB activation

Key Product Details

Description:	Proteasome and calpain inhibitor. Inhibits NF-κB activation
Alternative Names:	Z-LLL-al,Z-Leu-Leu-Leu-CHO
Chemical Name:	N-[(Phenylmethoxy)carbonyl]-L-leucyl-N-[(1S)-1-formyl-3-methylbutyl]-L-leucinamide
Purity:	≥95% (HPLC)
Molecular Weight:	475.63

View Full Technical Details for MG 132

Technical Data
Citations / Reviews (4)

Biological Activity for MG 132

MG 132 is a potent cell-permeable inhibitor of proteasome (IC₅₀ = 100 nM) and calpain (IC₅₀ = 1.2 μM). MG 132 inhibits TNF-α-induced NF-κB activation and IκBα degradation. Induces neurite outgrowth in PC12 cells and has anticancer properties in vitro. MG 132 inhibits SARS-CoV-2 M^pro in vitro (IC₅₀ = 3.9 μM) and inhibits viral replication. Also enhances adeno-associated viral transduction efficiency of human cell lines by around 50-fold.

Technical Data for MG 132

M. Wt	475.63
Formula	C₂₆H₄₁N₃O₅
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	133407-82-6
PubChem ID	90488707
InChi Key	AUQBNOXQYSNYAH-OJSMNCEXSA-N
Smiles	[H]C(C)C[C@H](NC(=O)OCC1=CC=CC=C1)C(=O)NC(CC(C)C)C(=O)N[C@@H](CC(C)C)C(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for MG 132

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	47.56	100
	DMSO	47.56	100

Preparing Stock Solutions for MG 132

The following data is based on the product molecular weight 475.63.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.10 mL	10.51 mL	21.02 mL
5 mM	0.42 mL	2.10 mL	4.20 mL
10 mM	0.21 mL	1.05 mL	2.10 mL
50 mM	0.04 mL	0.21 mL	0.42 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for MG 132

References are publications that support the biological activity of the product.

Douar Intracellular trafficking of adeno-associated virus vectors: routing to the late endosomal compartment and proteasome degradation J.Virol. 2001 PMID: 11160681
Ma Boceprevir, GC-376, and calpain inhibitors II, XII inhibit SARS-CoV-2 viral replication by targeting the viral main protease. Cell Res. 2020 PMID: 32541865
Palombella The ubiquitin-proteasome pathway is required for processing the NF-κB1 precursor protein and the activation of NF-κB. Cell 1994 PMID: 8087845
Tsubuki Differential inhibition of calpain and proteasome activities by peptidyl aldehydes of di-Leucine and tri-Leucine. J.Biochem. 1996 PMID: 8830056
Banerjee and Liefshitz Potential of the proteasome inhibitor MG-132 as an anticancer agent, alone and in combination. Anticancer Res. 2001 PMID: 11911275