Monastrol

Name: Monastrol
Brand: Tocris Bioscience
SKU: 1305

Tocris Bioscience, part of Bio-Techne | Catalog # 1305

Citations (78)

COA / SDS

Selective inhibitor of mitotic kinesin Eg5

Key Product Details

Description:	Selective inhibitor of mitotic kinesin Eg5
Chemical Name:	1,2,3,4-Tetrahydro-4-(3-hydroxyphenyl)-6-methyl-2-thioxo-5-pyrimidinecarboxylic acid, ethyl ester
Purity:	≥98% (HPLC)
Molecular Weight:	292.35

View Full Technical Details for Monastrol

Technical Data
Citations

Biological Activity for Monastrol

Monastrol is a potent, cell-permeable, small molecule mitosis inhibitor that does not interact with tubulin. Arrests cells in mitosis and specifically inhibits the motility of the mitotic kinesin Eg5, a motor protein required for mitotic spindle formation and maintenance (IC₅₀ = 14 μM).

Licensing Information

Sold with the permission of Harvard University

Compound Libraries for Monastrol

Monastrol is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Monastrol

M. Wt	292.35
Formula	C₁₄H₁₆N₂O₃S
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	254753-54-3
PubChem ID	5353454
InChi Key	UAIYNMRLUHHRMF-AATRIKPKSA-N
Smiles	CCOC(=O)C1=C(C)NC(=S)NC1C1=CC(O)=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Monastrol

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	5.85	20
	DMSO	29.24	100

Preparing Stock Solutions for Monastrol

The following data is based on the product molecular weight 292.35.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.42 mL	17.10 mL	34.21 mL
5 mM	0.68 mL	3.42 mL	6.84 mL
10 mM	0.34 mL	1.71 mL	3.42 mL
50 mM	0.07 mL	0.34 mL	0.68 mL

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References for Monastrol

References are publications that support the biological activity of the product.

Mayer Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. Science 1999 PMID: 10542155
Kapoor Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5. J.Cell Biol. 2000 PMID: 10973989