Mpro 13b-K

Name: Mpro 13b-K
Brand: Tocris Bioscience
SKU: 7228

Tocris Bioscience, part of Bio-Techne | Catalog # 7228

Citations (2)

COA / SDS

Coronavirus Mpro inhibitor

Key Product Details

Description:	Coronavirus M^pro inhibitor
Chemical Name:	tert-Butyl (1-((S)-1-(((S)-4-(benzylamino)-3,4-dioxo-1-((S)-2-oxopyrrolidin-3-yl)butan-2-yl)amino)-3-cyclopropyl-1-oxopropan-2-yl)-2-oxo-1,2-dihydropyridin-3-yl)carbamate
Purity:	≥97% (HPLC)
Molecular Weight:	593.68

View Full Technical Details for Mpro 13b-K

Technical Data
Citations

Biological Activity for Mpro 13b-K

Mpro 13b-K is a coronavirus M^pro inhibitor (IC₅₀ values are 0.12, 0.58 and 0.9 μM for recombinant M^pro from SARS-CoV-2, MERS-CoV and SARS-CoV, respectively). Mpro 13b-K inhibits SARS-CoV-2 infection of human Calu3 cells (IC₅₀ = 2.4 μM). The compound also inhibits SARS-CoV-2 replication in A549-ACE2-TMPRSS2, VeroE6 and Huh7 cell cultures (EC₅₀ values are 0.84, 1.3 and 3.4 μM). Exhibits oral and inhalative bioavailability.

Please note that this product was previously known as Mpro 13b.

Licensing Information

Sold under license from the University of Lübeck

Technical Data for Mpro 13b-K

M. Wt	593.68
Formula	C₃₁H₃₉N₅O₇
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	2412965-59-2
PubChem ID	146026181
InChi Key	NLVRHQFXQFSBQK-XWGVYQGASA-N
Smiles	CC(C)(OC(NC1=CC=CN(C1=O)[C@H](C(N[C@H](C(C(NCC2=CC=CC=C2)=O)=O)C[C@@H]3CCNC3=O)=O)CC4CC4)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Mpro 13b-K

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	59.37	100

Preparing Stock Solutions for Mpro 13b-K

The following data is based on the product molecular weight 593.68.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.68 mL	8.42 mL	16.84 mL
5 mM	0.34 mL	1.68 mL	3.37 mL
10 mM	0.17 mL	0.84 mL	1.68 mL
50 mM	0.03 mL	0.17 mL	0.34 mL

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References for Mpro 13b-K

References are publications that support the biological activity of the product.

Cooper Diastereomeric resolution yields highly potent inhibitor of SARS-CoV-2 main protease. J.Med.Chem. 2022 PMID: 36179320
Zhang Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved α-ketoamide inhibitors. Science 2020 PMID: 32198291