Nexinhib20

Name: Nexinhib20
Brand: Tocris Bioscience
SKU: 6089

Tocris Bioscience, part of Bio-Techne | Catalog # 6089

Citations (9)

COA / SDS

Rab27 inhibitor; inhibits neutrophil exocytosis; active in vivo

Key Product Details

Description:	Rab27 inhibitor; inhibits neutrophil exocytosis; active in vivo
Chemical Name:	4,4-Dimethyl-1-(3-nitrophenyl)-2-(1H-1,2,4-triazol-1-yl)-1-penten-3-one
Purity:	≥98% (HPLC)
Molecular Weight:	300.31

View Full Technical Details for Nexinhib20

Technical Data
Citations

Biological Activity for Nexinhib20

Nexinhib20 is a Rab27 inhibitor (IC₅₀ = 330 nM); inhibits Rab27a-JFC1 binding. Selectively inhibits neutrophil exocytosis, with no significant effect on other important innate immune responses including phagocytosis. Suppresses extracellular production of superoxide anion and cytochrome b plasma membrane expression. Also inhibits upregulation of adhesion molecules CD11b and CD66b. Displays in vivo anti-inflammatory activity.

Compound Libraries for Nexinhib20

Nexinhib20 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Nexinhib20

M. Wt	300.31
Formula	C₁₅H₁₆N₄O₃
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	331949-35-0
PubChem ID	5715188
InChi Key	VKSOIYNNOFGGES-JYRVWZFOSA-N
Smiles	CC(C)(C(/C(N1C=NC=N1)=C/C2=CC([N+]([O-])=O)=CC=C2)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Nexinhib20

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	30.03	100

Preparing Stock Solutions for Nexinhib20

The following data is based on the product molecular weight 300.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.33 mL	16.65 mL	33.30 mL
5 mM	0.67 mL	3.33 mL	6.66 mL
10 mM	0.33 mL	1.66 mL	3.33 mL
50 mM	0.07 mL	0.33 mL	0.67 mL

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References for Nexinhib20

References are publications that support the biological activity of the product.

Johnson Identification of neutrophil exocytosis inhibitors (Nexinhibs), small molecule inhibitors of neutrophil exocytosis and inflammation: druggability of the small GTPase Rab27a. J.Biol.Chem. 2016 PMID: 27702998