NFkB p50 (NLS) Inhibitor Peptide Set
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29323
Key Product Details
Species
Human, Mouse, Rat, Bovine, Canine, Chicken, Xenopus
Applications
Inhibition Activity
Product Specifications
Specificity
The p50 NLS inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable.
The control peptide consists of only the PTD sequence
The control peptide consists of only the PTD sequence
Applications
Functional (Inhibition) (reported in scientific literature (PMID 24384074))
Application Notes
Inhibition of NF-kB p50(NLS) activity. The inhibitor peptide is designed to block p50 nuclear translocation. Optimal peptide concentrations and incubation times vary between model systems and should be determined empirically by users. A 100 uM final concentration may be a useful starting point. Please refer to Lin et al (1995) for additional information about how the inhibitor peptide has been used to block p50 nuclear translocation.
Reactivity Notes
Broad: Peptide sequence is 100% conserved across multiple species.
Inhibitor Content
NF-kB p50 (NLS) Inhibitor peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKKVQRKRQKLM (p50 NLS sequence: VQRKRQKLM). Molecular weight: 3529.
Antennapedia Control peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361
Scientific Data Images for NFkB p50 (NLS) Inhibitor Peptide Set
![Functional (Inhibition): NFkB p50 (NLS) Inhibitor Peptide Set [NBP2-29323]](https://resources.bio-techne.com/images/products/NFkB-p105-p50-Inhibitor-Peptide-Set-Functional-Inhibition-NBP2-29323-img0001.jpg "Functional (Inhibition): NFkB p50 (NLS) Inhibitor Peptide Set [NBP2-29323]")
Functional (Inhibition): NFkB p50 (NLS) Inhibitor Peptide Set [NBP2-29323]
Functional (Inhibition): NFkB p105/p50 Inhibitor Peptide Set [NBP2-29323] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29323) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Flagellin (NBP2-25289) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).Formulation, Preparation, and Storage
Preparation Method
Preparation of 5 mM Stock Solutions
PBS* is added directly to the vials to prepare the stock solutions.
Note: Bring the solution to room temperature and quick spin the tubes before opening the caps. p50 (NLS)
Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKVQRKRQKLM
Add 57 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.
Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at 20C or -80C. Avoid repeated freeze thawing.
Recipe for 1X PBS:
1. Dissolve the following in 800ml distilled H2O.
- 8g of NaCl
- 0.2g of KCl
- 1.44g of Na2HPO4
- 0.24g of KH2PO4
2. Adjust pH to 7.5 with HCl.
3. Adjust volume to 1L with additional distilled H2O.
4. Sterilize by autoclaving
PBS* is added directly to the vials to prepare the stock solutions.
Note: Bring the solution to room temperature and quick spin the tubes before opening the caps. p50 (NLS)
Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKVQRKRQKLM
Add 57 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.
Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at 20C or -80C. Avoid repeated freeze thawing.
Recipe for 1X PBS:
1. Dissolve the following in 800ml distilled H2O.
- 8g of NaCl
- 0.2g of KCl
- 1.44g of Na2HPO4
- 0.24g of KH2PO4
2. Adjust pH to 7.5 with HCl.
3. Adjust volume to 1L with additional distilled H2O.
4. Sterilize by autoclaving
Concentration
Lyoph
Reconstitution Instructions
Please contact technical support for detailed reconstitution instructions.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NFkB p50 (NLS)
Alternate Names
CVID12, EBP-1, KBF1, NFkappaB, NF-kappaB, NF-kappa-B1, NFKB1, NF-kB1, NFKB-p105, NFKB-p50, nuclear factor kappa B subunit 1, p105, P50
Gene Symbol
NFKB1
Additional NFkB p50 (NLS) Products
Product Documents for NFkB p50 (NLS) Inhibitor Peptide Set
Product Specific Notices for NFkB p50 (NLS) Inhibitor Peptide Set
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.
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