NICE 01

Name: NICE 01
Brand: Tocris Bioscience
SKU: 8088

Tocris Bioscience, part of Bio-Techne | Catalog # 8088

COA / SDS

Heterobifunctional compound for nuclear import of FKBPF36V tagged proteins

Key Product Details

Description:	Heterobifunctional compound for nuclear import of FKBP^F36V tagged proteins
Chemical Name:	(R)-1-(3-((14-((S)-4-(4-Chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-2,13-dioxo-6,9-dioxa-3,12-diazatetradecyl)oxy)phenyl)-3-(3,4-dimethoxyphenyl)propyl (S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate
Purity:	≥98% (HPLC)
Molecular Weight:	1206.85

View Full Technical Details for NICE 01

Technical Data
Citations

Biological Activity for NICE 01

NICE 01 is a bifunctional molecule consisting of (+)-JQ1 (Cat. No. 4499) and AP 1867 (Cat. No. 6207), with a PEG2-diamine linker. It has a high affinity (K_d = 94 pM) for FKBP^F36V tagged proteins. It can induce nuclear localization of cytosolic proteins and target transcriptional regulation. It can be used to calculate the kinetic constant and passive diffusion across the nuclear pore.

Technical Data for NICE 01

M. Wt	1206.85
Formula	C₆₃H₇₆ClN₇O₁₃S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2982819-94-1
PubChem ID	169183208
InChi Key	USCBOLCYUGPYAF-YUZLDYPQSA-N
Smiles	O=C(O[C@H](CCC1=CC=C(C(OC)=C1)OC)C2=CC=CC(OCC(NCCOCCOCCNC(C[C@H]3C4=NN=C(N4C5=C(C(C6=CC=C(C=C6)Cl)=N3)C(C)=C(S5)C)C)=O)=O)=C2)[C@H]7N(CCCC7)C([C@@H](CC)C8=CC(OC)=C(C(OC)=C8)OC)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NICE 01

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	60.34	50

Preparing Stock Solutions for NICE 01

The following data is based on the product molecular weight 1206.85.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	1.66 mL	8.29 mL	16.57 mL
2.5 mM	0.33 mL	1.66 mL	3.31 mL
5 mM	0.17 mL	0.83 mL	1.66 mL
25 mM	0.03 mL	0.17 mL	0.33 mL

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References for NICE 01

References are publications that support the biological activity of the product.

Gibson Bifunctional small molecules that induce nuclear localization and targeted transcriptional regulation. J.Am.Chem.Soc. 2023 PMID: 37461636
Lu Bioorthogonal chemical epigenetic modifiers enable dose-dependent CRISPR targeted gene activation in mammalian cells. A.C.S.Synth.Biol. 2022 PMID: 35302756