Nimbolide

Name: Nimbolide
Brand: Tocris Bioscience
SKU: 7289

Tocris Bioscience, part of Bio-Techne | Catalog # 7289

Citations (1)

COA / SDS

RNF114 inhibitor

Key Product Details

Description:	RNF114 inhibitor
Chemical Name:	(2aR,5aR,6S,6aR,8R,9aR,10aS,10bR,10cR)-8-(3-Furanyl)-2a,5a,6,6a,8,9,9a,10a,10b,10c-decahydro-2a,5a,6a,7-tetramethyl-2,5-dioxo-2H,5H-cyclopenta[d']naphtho[1,8-bc:2,3-b']difuran-6-acetic acid methyl ester
Purity:	≥97% (HPLC)
Molecular Weight:	466.53

View Full Technical Details for Nimbolide

Technical Data
Citations

Biological Activity for Nimbolide

Nimbolide inhibits auto-ubiquitination of the E3 ligase RNF114 and p21 ubiquitination in vitro. This compound impairs proliferation of triple-negative breast cancer cell lines. Nimbolide also induces apoptotic cell death in a Waldenströms macroglobulinemia cell line (BCWM1) and inhibits tumor growth in a xenograft model of Waldenströms macroglobulinemia.

Nimbolide can be used for the development of Degraders that utilize RNF114 as an E3 ligase for Targeted Protein Degradation.

Technical Data for Nimbolide

M. Wt	466.53
Formula	C₂₇H₃₀O₇
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	25990-37-8
PubChem ID	12313376
InChi Key	JZIQWNPPBKFOPT-LSYMHUITSA-N
Smiles	[H][C@@]12C[C@@H](C3=COC=C3)C(C)=C1[C@]4([C@@H]([C@]5(C(C=C[C@]6(C(O[C@@]([C@]4(O2)[H])([C@]56[H])[H])=O)C)=O)C)CC(OC)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Nimbolide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	46.65	100

Preparing Stock Solutions for Nimbolide

The following data is based on the product molecular weight 466.53.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.14 mL	10.72 mL	21.43 mL
5 mM	0.43 mL	2.14 mL	4.29 mL
10 mM	0.21 mL	1.07 mL	2.14 mL
50 mM	0.04 mL	0.21 mL	0.43 mL

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References for Nimbolide

References are publications that support the biological activity of the product.

Spradin Harnessing the anti-cancer natural product nimbolide for targeted protein degradation. Nat.Chem.Biol. 2019 PMID: 31209351
Tong A nimbolide-based kinase degrader preferentially degrades oncogenic BCR-ABL. ACS Chem.Biol. 2020 PMID: 32568522
Chitta Nimbolide targets BCL2 and induces apoptosis in preclinical models of Waldenströms macroglobulinemia. Blood Cancer J. 2014 PMID: 25382610