NSC 663284

Name: NSC 663284
Brand: Tocris Bioscience
SKU: 1867

Tocris Bioscience, part of Bio-Techne | Catalog # 1867

Citations (6)

COA / SDS

Potent, selective Cdc25 phosphatase inhibitor

Key Product Details

Description:	Potent, selective Cdc25 phosphatase inhibitor
Chemical Name:	6-Chloro-7-[[2-(4-morpholinyl)ethyl]amino]-5,8-quinolinedione
Purity:	≥99% (HPLC)
Molecular Weight:	321.76

View Full Technical Details for NSC 663284

Technical Data
Citations

Biological Activity for NSC 663284

NSC 663284 is a potent, selective inhibitor of Cdc25 dual specificity phosphatases (K_i values are 29, 95 and 89 nM for human Cdc25A, Cdc25B₂ and Cdc25C respectively); > 20- and > 450-fold selective over VHR and PTP1B phosphatases respectively. Arrests cells at both G₁ and G₂/M phase and blocks cdk2 and cdk1 activation. Blocks proliferation of a range of human tumor cell lines (IC₅₀ = 0.2 - 35 μM).

Compound Libraries for NSC 663284

NSC 663284 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for NSC 663284

M. Wt	321.76
Formula	C₁₅H₁₆ClN₃O₃
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	383907-43-5
PubChem ID	379077
InChi Key	BMKPVDQDJQWBPD-UHFFFAOYSA-N
Smiles	ClC1=C(NCCN2CCOCC2)C(=O)C2=NC=CC=C2C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NSC 663284

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	16.1	50
	ethanol	16.1	50

Preparing Stock Solutions for NSC 663284

The following data is based on the product molecular weight 321.76.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	6.22 mL	31.08 mL	62.16 mL
2.5 mM	1.24 mL	6.22 mL	12.43 mL
5 mM	0.62 mL	3.11 mL	6.22 mL
25 mM	0.12 mL	0.62 mL	1.24 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for NSC 663284

References are publications that support the biological activity of the product.

Lazo Discovery and biological evaluation of a new family of potent inhibitors of the dual specificity protein phosphatase Cdc25. J.Med.Chem. 2001 PMID: 11708908
Pu Dual G₁ and G₂ phase inhibition by a novel, selective Cdc25 inhibitor 7-chloro-6-(2-morpholin-4-ylethylamino)-quinoline-5,8-dione. J.Biol.Chem. 2002 PMID: 12356752
Brisson Redox regulation of cdc25B by cell-active Quinolinediones. Mol.Pharmacol. 2005 PMID: 16155209