PARPYnD

Name: PARPYnD
Brand: Tocris Bioscience
SKU: 7410

Tocris Bioscience, part of Bio-Techne | Catalog # 7410

COA / SDS

PARP inhibitor; photoaffinity probe

Key Product Details

Description:	PARP inhibitor; photoaffinity probe
Chemical Name:	3-(3-Butyn-1-yl)-N-[5-cyano-6-[4-[3-[(3,4-dihydro-4-oxo-1-phthalazinyl)methyl]benzoyl]-1-piperazinyl]-3-pyridinyl]-3H-diazirine-3-propanamide
Purity:	≥95% (HPLC)
Molecular Weight:	613.68

View Full Technical Details for PARPYnD

Technical Data
Citations

Biological Activity for PARPYnD

PARPYnD is a potent photoaffinity probe (AfBP) for poly(ADP-ribose) polymerase (PARP) (IC₅₀ values for PARP2, PARP1 and PARP6 are 6, 38 and 230 nM, respectively). Can be used to fluorescently label PARP1 and PARP2 in cells when an azide functionalized probe is attached via copper catalysed click-chemistry. PARPYnD enriches recombinant PARP6 spiked into cellular lysates and inhibits PARP6 in cell-free assays; does not label PARP6 in intact cells. Photocrosslinking can be performed by irradiating at 365 nm.

Technical Data for PARPYnD

M. Wt	613.68
Formula	C₃₄H₃₁N₉O₃
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	2561483-27-8
InChi Key	ZYWZRMXOMDKHKX-UHFFFAOYSA-N
Smiles	O=C(NC1=CN=C(C(C#N)=C1)N2CCN(C(C3=CC(CC4=NNC(C5=CC=CC=C45)=O)=CC=C3)=O)CC2)CCC6(N=N6)CCC#C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PARPYnD

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	61.37	100

Preparing Stock Solutions for PARPYnD

The following data is based on the product molecular weight 613.68.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.63 mL	8.15 mL	16.30 mL
5 mM	0.33 mL	1.63 mL	3.26 mL
10 mM	0.16 mL	0.81 mL	1.63 mL
50 mM	0.03 mL	0.16 mL	0.33 mL

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References for PARPYnD

References are publications that support the biological activity of the product.

Howard Structure-guided design and in-cell target profiling of a cell-active target engagement probe for PARP inhibitors. ACS Chem.Biol. 2020 PMID: 32017532