PD 407824

Name: PD 407824
Brand: Tocris Bioscience
SKU: 2694

Tocris Bioscience, part of Bio-Techne | Catalog # 2694

Citations (11)

COA / SDS

Potent and selective inhibitor of Chk1 and Wee1

Key Product Details

Description:	Potent and selective inhibitor of Chk1 and Wee1
Chemical Name:	9-Hydroxy-4-phenyl-pyrrolo[3,4-c]carbazole-1,3(2H,6H)-dione
Purity:	≥98% (HPLC)
Molecular Weight:	328.32

View Full Technical Details for PD 407824

Technical Data
Citations

Biological Activity for PD 407824

PD 407824 is a potent and selective inhibitor of checkpoint kinases Chk1 and Wee1 (IC₅₀ values are 47 and 97 nM respectively). Displays selectivity over a range of other protein kinases; IC₅₀ values are 3.4, 3.75, > 5, > 50, > 50 and > 50 μM for PKC, CDK4, other CDKs, c-Src, PDGFR and FGFR respectively. Also sensitizes hESCs to BMP4 to induce differentiation.

Licensing Information

Sold for research purposes under agreement from Pfizer Inc.

Compound Libraries for PD 407824

PD 407824 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for PD 407824

M. Wt	328.32
Formula	C₂₀H₁₂N₂O₃
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	622864-54-4
PubChem ID	4369491
InChi Key	IAUZTOZLTFSMIE-UHFFFAOYSA-N
Smiles	OC1=CC2=C(NC3=C2C2=C(C(=O)NC2=O)C(=C3)C2=CC=CC=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PD 407824

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	32.83	100
	ethanol	8.2	25

Preparing Stock Solutions for PD 407824

The following data is based on the product molecular weight 328.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.05 mL	15.23 mL	30.46 mL
5 mM	0.61 mL	3.05 mL	6.09 mL
10 mM	0.30 mL	1.52 mL	3.05 mL
50 mM	0.06 mL	0.30 mL	0.61 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for PD 407824

References are publications that support the biological activity of the product.

Palmer 4-Phenylpyrrolo[3,4-c]carbazole-1,3(2H,6H)-dione inhibitors of the checkpoint kinase Wee1. Structure-activity relationships for chromophore modification and phenylring substitutions. J.Med.Chem. 2006 PMID: 16884302
Feng Discovery of a small-molecule BMP sensitizer for human embryonic stem cell differentiation. Cell Rep. 2016 PMID: 27210748
Squire Structure and inhibition of the human cell cycle checkpoint kinase, Wee1A kinase: an atypical tyrosine kinase with a key role in CDK1 regulation. Structure 2005 PMID: 15837193