Phenamil

Name: Phenamil
Brand: Tocris Bioscience
SKU: 3379

Tocris Bioscience, part of Bio-Techne | Catalog # 3379

Citations (3)

COA / SDS

Inhibits TRPP3-mediated currents; also inhibits epithelial Na+ channels

Key Product Details

Description:	Inhibits TRPP3-mediated currents; also inhibits epithelial Na⁺ channels
Chemical Name:	3,5-Diamino-6-chloro-N-[imino(phenylamino)methyl]pyrazinecarboxamide methanesulfonate salt
Purity:	≥99% (HPLC)
Molecular Weight:	401.83

View Full Technical Details for Phenamil

Technical Data
Citations

Biological Activity for Phenamil

Phenamil is a TRPP3 channel inhibitor (IC₅₀ = 0.14 μM). Also inhibits epithelial Na⁺ channels (K_d = 0.4 nM for a high affinity site on the epithelial Na⁺ channel). Derivative of amiloride (Cat. No. 0890).

Compound Libraries for Phenamil

Phenamil is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Phenamil

M. Wt	401.83
Formula	C₁₂H₁₂ClN₇O.CH₃SO₃H
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	1161-94-0
PubChem ID	9604979
InChi Key	MHPIZTURFVSLTJ-UHFFFAOYSA-N
Smiles	NC1=C(Cl)N=C(C(NC(NC2=CC=CC=C2)=N)=O)C(N)=N1.CS(=O)(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Phenamil

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	40.18	100

Preparing Stock Solutions for Phenamil

The following data is based on the product molecular weight 401.83.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.49 mL	12.44 mL	24.89 mL
5 mM	0.50 mL	2.49 mL	4.98 mL
10 mM	0.25 mL	1.24 mL	2.49 mL
50 mM	0.05 mL	0.25 mL	0.50 mL

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Reconstitution Calculator

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References for Phenamil

References are publications that support the biological activity of the product.

Dai Inhibition of TRPP3 channel by amil. and analogs. Mol.Pharmacol. 2007 PMID: 17804601
Barbry Biochemical identification of two types of phenamil binding sites associated with amiloride-sensitive Na⁺ channels. Biochemistry. 1989 PMID: 2546581