Psoralen-triethylene glycol azide

Name: Psoralen-triethylene glycol azide
Brand: Tocris Bioscience
SKU: 6862

Tocris Bioscience, part of Bio-Techne | Catalog # 6862

COA / SDS

RNA conformation probe; reagent for COMRADES RNA platform

Key Product Details

Description:	RNA conformation probe; reagent for COMRADES RNA platform
Alternative Names:	psoralen-triethylene glycol azide
Chemical Name:	3-(13-Azido-5,8,11-trioxa-2-azatridec-1-yl)-2,5,9-trimethyl-7H-furo[3,2-g][1]benzopyran-7-one
Purity:	≥98% (HPLC)
Molecular Weight:	458.52

View Full Technical Details for Psoralen-triethylene glycol azide

Technical Data
Citations

Biological Activity for Psoralen-triethylene glycol azide

Psoralen TEG Azide is used to probe RNA structure and conformation in live cells, using the cross-linking of matched RNAs and deep sequencing or COMRADES method. Psoralen crosslinks the RNA and the azide enables subsequent click reaction with biotin. The COMRADES method has been used to determine the architecture of the Zika virus RNA genome inside cells, and identified multiple site-specific interactions with human noncoding RNAs. It has also been used to map RNA-RNA interactions along the SARS-CoV-2 genome and subgenomes inside cells. Psoralen TEG Azide is cell permeable. For detailed methods please see references section below.

Technical Data for Psoralen-triethylene glycol azide

M. Wt	458.52
Formula	C₂₃H₃₀N₄O₆
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1352815-11-2
InChi Key	LVWBLKATXVGKHP-UHFFFAOYSA-N
Smiles	O=C(C=C1C)OC2=C1C=C(C(CNCCOCCOCCOCCN=[N+]=[N-])=C(C)O3)C3=C2C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Psoralen-triethylene glycol azide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	45.85	100
	ethanol	22.93	50

Preparing Stock Solutions for Psoralen-triethylene glycol azide

The following data is based on the product molecular weight 458.52.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.18 mL	10.90 mL	21.81 mL
5 mM	0.44 mL	2.18 mL	4.36 mL
10 mM	0.22 mL	1.09 mL	2.18 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

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References for Psoralen-triethylene glycol azide

References are publications that support the biological activity of the product.

Ziv COMRADES determines in vivo RNA structures and interactions. Nat. Methods 2018 PMID: 30202058
Ziv The short- and long-range RNA-RNA interactome of SARS-CoV-2. Mol. Cell 2020 PMID: 33259809