Puromycin dihydrochloride

Name: Puromycin dihydrochloride
Brand: Tocris Bioscience
SKU: 4089
Rating: 4 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4089

(1)

Citations (25)

COA / SDS

Protein synthesis inhibitor

Key Product Details

Description:	Protein synthesis inhibitor
Chemical Name:	3'-[α-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	544.43

View Full Technical Details for Puromycin dihydrochloride

Technical Data
Citations / Reviews (1)

Biological Activity for Puromycin dihydrochloride

Puromycin dihydrochloride is a protein synthesis inhibitor; leads to the premature release of polypeptide chains as polypeptidyl purine derivatives. Analog of the 3' end of aminoacyl-tRNA. Aminonucleoside antibiotic. Inhibits translation in both in vitro and in vivo systems. Also inhibits the transport of proteins into the mitochondria in vitro.

Technical Data for Puromycin dihydrochloride

M. Wt	544.43
Formula	C₂₂H₂₉N₇O₅.2HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	58-58-2
PubChem ID	443311
InChi Key	MKSVFGKWZLUTTO-FZFAUISWSA-N
Smiles	O[C@@H]1[C@H](NC([C@@H](N)CC4=CC=C(OC)C=C4)=O)[C@@H](CO)O[C@H]1N3C2=NC=NC(N(C)C)=C2N=C3.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Puromycin dihydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	54.44	100
	DMSO	54.44	100

Preparing Stock Solutions for Puromycin dihydrochloride

The following data is based on the product molecular weight 544.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.84 mL	9.18 mL	18.37 mL
5 mM	0.37 mL	1.84 mL	3.67 mL
10 mM	0.18 mL	0.92 mL	1.84 mL
50 mM	0.04 mL	0.18 mL	0.37 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Puromycin dihydrochloride

References are publications that support the biological activity of the product.

Azzam and Algranati Mechanism of puromycin action: fate of ribosomes after release of nascent polypeptide chains from polysomes. Proc.Nat.Acad.Sci. 1973 PMID: 4590173
Price and Verner Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction. Biochim.Biophys.Acta. 1993 PMID: 8334141