RF 9

Tocris Bioscience, part of Bio-Techne | Catalog # 3672

COA / SDS

Selective NPFF1 and NPFF2 antagonist

Key Product Details

Description:	Selective NPFF₁ and NPFF₂ antagonist
Purity:	≥95% (HPLC)
Molecular Weight:	482.62

View Full Technical Details for RF 9

Technical Data
Citations

Biological Activity for RF 9

RF 9 is a selective NPFF receptor antagonist (K_i values are 58 and 75 nM at hNPFF₁ and hNPFF₂ receptors respectively). Displays selectivity over several related receptors including NPY Y₁ GPR10, GPR54, GPR103 and the opioid receptors. Blocks NPFF-induced hypothermia and increases in atrial blood pressure and heart rate, and prevents opioid-induced tolerance associated with hyperalgesia in vivo.

Technical Data for RF 9

M. Wt	482.62
Formula	C₂₆H₃₈N₆O₃
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	876310-60-0
PubChem ID	53320361
InChi Key	UMKHUSRDQFQHAK-RNJMTYCLSA-N
Smiles	O=C(N[C@H]([C@](N[C@H](C(N)=O)CC4=CC=CC=C4)=O)CCCNC(N)=N)C1(CC(C3)C2)CC3CC2C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for RF 9

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	4.83	10

Preparing Stock Solutions for RF 9

The following data is based on the product molecular weight 482.62.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	20.72 mL	103.60 mL	207.20 mL
0.5 mM	4.14 mL	20.72 mL	41.44 mL
1 mM	2.07 mL	10.36 mL	20.72 mL
5 mM	0.41 mL	2.07 mL	4.14 mL

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References for RF 9

References are publications that support the biological activity of the product.

Simonin RF9, a potent and selective neuropeptide FF receptor antagonist, prevents opioid-induced tolerance associated with hyperalgesia. Proc.Natl.Acad.Sci.USA
Fang Inhibition of neuropeptide FF (NPFF)-induced hypothermia and anti-morphine analgesia by RF9, a new selective NPFF receptors antagonist. Regul.Peptides
Wang Neuropeptide FF receptors antagonist, RF9, attenuates opioid-evoked hypothermia in mice. Peptides 2008 PMID: 18406009