SB 218078

Name: SB 218078
Brand: Tocris Bioscience
SKU: 2560

Tocris Bioscience, part of Bio-Techne | Catalog # 2560

(1)

Citations (33)

COA / SDS

Chk1 inhibitor

Key Product Details

Chemical Name:	9,10,11,12-Tetrahydro- 9,12-epoxy-1H-diindolo[1,2,3-fg:3',2',1'-kl]pyrrolo[3,4-i][1,6]benzodiazocine-1,3(2H)-dione
Purity:	≥98%
Molecular Weight:	393.39 kDa

View Full Technical Details for SB 218078

Technical Data
Citations / Reviews (1)

Biological Activity for SB 218078

SB 218078 is an inhibitor of checkpoint kinase 1 (Chk1) that displays selectivity over other protein kinases (IC₅₀ values are 15, 250 and 1000 nM for Chk1, cdc2 and PKC respectively). Abrogates G₂ cell cycle arrest caused by γ-irradiation and topoisomerase I inhibition. Potentiates cytotoxicity of DNA-damaging drugs, enhancing the efficacy of some chemotherapeutics.

Technical Data for SB 218078

M. Wt	393.39 kDa
Formula	C₂₄H₁₅N₃O₃
Storage	Store at RT
Purity	≥98%
CAS Number	135897-06-2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SB 218078

Solubility

Soluble Soluble to 100 mM in DMSO

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for SB 218078

References are publications that support the biological activity of the product.

Pouliot L, Chen Y, Bai J, Guha R, Martin S, Gottesman M, Hall M Cisplatin sensitivity mediated by WEE1 and CHK1 is mediated by miR-155 and the miR-15 family. Cancer Res, 2012;72(22):5945-55. 2012 PMID: 22942255
Ward S, Kim H, Komurov K, Mendiratta S, Tsai P, Schmolke M, Satterly N, Manicassamy B, Forst C, Roth M, Garcia-Sastre A, Blazewska K, McKenna C, Fontoura B, White M Host modulators of H1N1 cytopathogenicity. PLoS ONE, 2012;7(8):e39284. 2012 PMID: 22876275
Jackson et al. An indolocarbazole inhibitor of human checkpoint kinase (Chk1) abrogates cell cycle arrest caused by DNA damage. Cancer Res., 2000;60:566 PMID: 10676638
Kawabe G₂ checkpoint abrogators as anticancer drugs. Mol.Cancer Ther., 2004;3:513 PMID: 15078995
Chen et al. Checkpoint kinase 1-mediated phosphorylation of cdc25C and bad proteins are involved in antitumor effects of loratadine-induced G₂/M phase cell-cycle arrest and apoptosis. Mol.Carcinogenesis, 2006;45:461