SB 4

Name: SB 4
Brand: Tocris Bioscience
SKU: 6881

Tocris Bioscience, part of Bio-Techne | Catalog # 6881

COA / SDS

Potent BMP4 agonist

Key Product Details

Description:	Potent BMP4 agonist
Chemical Name:	2-[[(4-Bromophenyl)methyl]thio]benzoxazole
Purity:	≥99% (HPLC)
Molecular Weight:	320.2

View Full Technical Details for SB 4

Technical Data
Citations

Biological Activity for SB 4

SB 4 is a potent BMP4 agonist (EC₅₀ = 74 nM). Activates canonical BMP signaling and increases SMAD-1/5/9 phosphorylation, with no effect on p-SMAD-2, p-SMAD-3 or p-TAK1. Bypasses noggin-induced inhibition of type I BMP receptors. When used in combination, SB 4 enhances BMP4 signaling and embryonic stem cell differentiation.

Compound Libraries for SB 4

SB 4 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for SB 4

M. Wt	320.2
Formula	C₁₄H₁₀BrNOS
Storage	Store at -20°C
Purity	≥99% (HPLC)
CAS Number	100874-08-6
PubChem ID	731364
InChi Key	JDVOIBFEFVKUPL-UHFFFAOYSA-N
Smiles	BrC1=CC=C(C=C1)CSC2=NC3=C(C=CC=C3)O2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SB 4

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	32.02	100
	ethanol	6.4	20

Preparing Stock Solutions for SB 4

The following data is based on the product molecular weight 320.2.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.12 mL	15.62 mL	31.23 mL
5 mM	0.62 mL	3.12 mL	6.25 mL
10 mM	0.31 mL	1.56 mL	3.12 mL
50 mM	0.06 mL	0.31 mL	0.62 mL

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Reconstitution Calculator

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References for SB 4

References are publications that support the biological activity of the product.

Hayaei Tehrani A comparison between BMP4 and SB4 in inducing germ line gene expression pattern during embryonic stem cells differentiation. Differentiation 2022 PMID: 34864442
Bradford High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds. J.Biol.Chem. 2019 PMID: 30602563