TASP 0277308

Name: TASP 0277308
Brand: Tocris Bioscience
SKU: 6498

Tocris Bioscience, part of Bio-Techne | Catalog # 6498

COA / SDS

Potent and selective S1P1 receptor antagonist; inhibits angiogenesis

Key Product Details

Description:	Potent and selective S1P₁ receptor antagonist; inhibits angiogenesis
Chemical Name:	3,4-Dichloro-N-[(1R)-1-[4-ethyl-5-[3-(4-methyl-1-piperazinyl)phenoxy]-4H-1,2,4-triazol-3-yl]ethyl]benzenesulfonamide
Purity:	≥98% (HPLC)
Molecular Weight:	539.48

View Full Technical Details for TASP 0277308

Technical Data
Citations

Biological Activity for TASP 0277308

TASP 0277308 is a potent and selective sphingosine 1-phosphate 1 (S1P₁) receptor antagonist (IC₅₀ = 7.8 nM). TASP 0277308 inhibits S1P-induced chemotaxis (IC₅₀ = 1.1-1.6 nM), HUVEC cell proliferation (IC₅₀ = 3-3.6 nM) and suppresses angiogenesis in vivo. TASP 0277308 also blocks VEGF-induced tube-formation of HUVECs in vitro and reverses or impairs the development of arthritis or mechano-allodynia.

Technical Data for TASP 0277308

M. Wt	539.48
Formula	C₂₃H₂₈Cl₂N₆O₃S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	945725-50-8
PubChem ID	56935569
InChi Key	MVOULGOLHZCXLD-MRXNPFEDSA-N
Smiles	CCN1C(OC2=CC(N3CCN(CC3)C)=CC=C2)=NN=C1[C@H](NS(=O)(C4=CC(Cl)=C(C=C4)Cl)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for TASP 0277308

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	2.7	5

Preparing Stock Solutions for TASP 0277308

The following data is based on the product molecular weight 539.48.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.05 mM	37.07 mL	185.36 mL	370.73 mL
0.25 mM	7.41 mL	37.07 mL	74.15 mL
0.5 mM	3.71 mL	18.54 mL	37.07 mL
2.5 mM	0.74 mL	3.71 mL	7.41 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for TASP 0277308

References are publications that support the biological activity of the product.

Fujii Blocking S1P interaction with S1P₁ receptor by a novel competitive S1P₁-selective antagonist inhibits angiogenesis. Biochem.Biophys.Res.Commun. 2012 PMID: 22387544
Fujii Amelioration of collagen-induced arthritis by a novel S1P₁ antagonist with immunomodulatory activities. J.Immunol. 2012 PMID: 22131329