TRAF-6 Inhibitor Peptide Set
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26506
Key Product Details
Species
Human, Mouse, Rat
Applications
Block/Neutralize, Functional Assay
Product Specifications
Specificity
The TRAF6 inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable. The control peptide consists of only the PTD sequence.
Applications
Block/Neutralize (reported in scientific literature (PMID 24829345))
In vitro assay (reported in scientific literature (PMID 24829345))
In vitro assay (reported in scientific literature (PMID 24829345))
Application Notes
Inhibition of RANKL mediated osteoclast differentiation.
Researchers can study the effect of TRAF6 inhibitor peptide using a variety of methods, such as EMSA, NF-kB/p65 ELISA,IkBa phosphorylation ELISA, osteoclast differentiation assay. The Osteoclast formation assay protocol is a guideline which may need to be optimized for different cell types.
Researchers can study the effect of TRAF6 inhibitor peptide using a variety of methods, such as EMSA, NF-kB/p65 ELISA,IkBa phosphorylation ELISA, osteoclast differentiation assay. The Osteoclast formation assay protocol is a guideline which may need to be optimized for different cell types.
Inhibitor Content
TRAF6 Inhibitor peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKKRKIPTEDEY (TRAF6 binding sequence: RKIPTEDEY). Molecular weight: 3494.
Antennapedia Control peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361.
Scientific Data Images for TRAF-6 Inhibitor Peptide Set
TRAF6 Inhibitor Peptide Set [NBP2-26506] - The TRAF6 inhibitory peptide inhibits osteoclast ifferentiation: RAW264.7 cells were plated on 24-well plates and stimulated with RANKL and M-CSF and incubated in the presence of different concentrations of control peptide (PTD) or the TRAF6. The TRAF6 at the concentration above 30 uM suppressed osteoclast differentiation.
Formulation, Preparation, and Storage
Preparation Method
Preparation of 5 mM Stock Solutions
PBS* is added directly to the vials to prepare the stock solutions. Note: Bring the solution to room temperature and quick spin the tubes before opening the caps.
TRAF6 Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKRKIPTEDEY
Add 57.7 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.
Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at 20C or -80C. Avoid repeated freeze thawing.
*Recipe for 1X PBS:
1. Dissolve the following in 800ml distilled H2O.
- 8g of NaCl
- 0.2g of KCl
- 1.44g of Na2HPO4
- 0.24g of KH2PO4
2. Adjust pH to 7.5 with HCl.
3. Adjust volume to 1L with additional distilled H2O.
4. Sterilize by autoclaving
PBS* is added directly to the vials to prepare the stock solutions. Note: Bring the solution to room temperature and quick spin the tubes before opening the caps.
TRAF6 Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKRKIPTEDEY
Add 57.7 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.
Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at 20C or -80C. Avoid repeated freeze thawing.
*Recipe for 1X PBS:
1. Dissolve the following in 800ml distilled H2O.
- 8g of NaCl
- 0.2g of KCl
- 1.44g of Na2HPO4
- 0.24g of KH2PO4
2. Adjust pH to 7.5 with HCl.
3. Adjust volume to 1L with additional distilled H2O.
4. Sterilize by autoclaving
Formulation
Form: White Solid
Solubilize the peptides prior to use by making 5 mM PBS* stock solutions (please see Preparation of 5 mM Stock Solutions under Preparation Method).
Solubilize the peptides prior to use by making 5 mM PBS* stock solutions (please see Preparation of 5 mM Stock Solutions under Preparation Method).
Concentration
Lyoph
Reconstitution Instructions
Please contact technical support for detailed reconstitution instructions.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TRAF-6
The TRAF6 inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable.The control peptide consists of only the PTD sequence
Long Name
TNF Receptor-Associated Factor 6
Alternate Names
TRAF6
Gene Symbol
TRAF6
Additional TRAF-6 Products
Product Documents for TRAF-6 Inhibitor Peptide Set
Product Specific Notices for TRAF-6 Inhibitor Peptide Set
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...