Wnt-C59

Name: Wnt-C59
Brand: Tocris Bioscience
SKU: 5148
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5148

(2)

Citations (105)

COA / SDS

Highly potent PORCN inhibitor

Key Product Details

Chemical Name:	4-(2-Methyl-4-pyridinyl)-N-[4-(3-pyridinyl)phenyl]benzeneacetamide
Purity:	≥99%

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Technical Data
Citations / Reviews (2)

Biological Activity for Wnt-C59

Wnt-C59 is a highly potent inhibitor of the MBOAT (membrane-bound O-acyltranferase) family member, Porcupine (PORCN) (IC₅₀ = 74 pM), that mediates WNT palmitoylation and secretion. Wnt-C59 potently inhibits the processing of both canonical (1, 2, 3a, 6, 7b, 8a, 9a, 9b, 10) and non-canonical (4, 5a, 11, 16) Wnt subtypes. Wnt-C59 blocks progression of mammary tumors in MMTV-WNT1 transgenic mice and downregulates Wnt/β-catenin target genes. Wnt-C59-treated tumors show a decrease in β-catenin, CyclinD1 and c-Myc. Wnt-C59 induces cardiomyocyte differentiation from human iPSCs following culture with CHIR 99021 (Cat. No. 4423). Wnt-C59 efficiently induces neural differentiation of CTIP2⁺/COUP-TF1^- cells from PSCs in culture. When grafted into the cortex of adult mice, Wnt-C59-treated cells develop abundant axonal fiber extensions toward the spinal cord. The compound has also been used in protocols to generate β cells from human PSCs. Cell permeable and orally bioavailable.

Technical Data for Wnt-C59

Formula	C₂₅H₂₁N₃O
Storage	Store at -20°C
Purity	≥99%
CAS Number	1243243-89-1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Wnt-C59

Solubility

Soluble Soluble to 20 mM in DMSO and to 20 mM in ethanol

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References for Wnt-C59

References are publications that support the biological activity of the product.

B Bourgeois, T Gui, D Hoogeboom, HG Hocking, G Richter, E Spreitzer, M Viertler, K Richter, T Madl, BMT Burgering Multiple regulatory intrinsically disordered motifs control FOXO4 transcription factor binding and function Cell Reports, 2021;36(4):109446. 2021 PMID: 34320339
RD Welch, C Billon, AC Valfort, TP Burris, CA Flaveny Pharmacological inhibition of REV-ERB stimulates differentiation, inhibits turnover and reduces fibrosis in dystrophic muscle Sci Rep, 2017;7(1):17142. 2017 PMID: 29215066
Burridge et al. Chemically defined generation of human cardiomyocytes. Nat.Methods, 2014;11:855 PMID: 24930130
Proffitt et al. Pharmacological inhibition of the Wnt acyltransferase PORCN prevents growth of WNT-driven mammary cancer. Cancer Res., 2013;73:502 PMID: 23188502
Youssef et al. Adult interfollicular tumour-initiating cells are reprogrammed into an embryonic hair follicle progenitor-like fate during basal cell carcinoma initiation. Nat. Cell Biol., 2012;14:1282 PMID: 23178882
Wend et al. WNT10B/β-catenin signalling induces HMGA2 and proliferation in metastatic triple-negative breast cancer. EMBO Mol.Med., 2013;5:264 PMID: 23307470

Wnt-C59

Tocris Bioscience, part of Bio-Techne | Catalog # 5148

Key Product Details

Biological Activity for Wnt-C59

Technical Data for Wnt-C59

Solubility for Wnt-C59

Dilution Calculator

Reconstitution Calculator

References for Wnt-C59

Product Documents for Wnt-C59

COA

Certificate of Analysis

SDS

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