Zebularine

Name: Zebularine
Brand: Tocris Bioscience
SKU: 2293

Tocris Bioscience, part of Bio-Techne | Catalog # 2293

Citations (11)

COA / SDS

DNA methyltransferase and cytidine deaminase inhibitor

Key Product Details

Description:	DNA methyltransferase and cytidine deaminase inhibitor
Alternative Names:	NSC 309132
Chemical Name:	1-β-D-Ribofuranosyl-2(1H)-pyrimidinone
Purity:	≥99% (HPLC)
Molecular Weight:	228.2

View Full Technical Details for Zebularine

Technical Data
Citations

Biological Activity for Zebularine

Zebularine is an orally active DNA methyltransferase inhibitor. Inhibits tumor cell proliferation (IC₅₀ = 120 μM) and re-activates silenced genes in T24 bladder carcinoma cells. Inhibits cytidine deaminase (K_i ~ 2 μM); induces differentiation of mesenchymal stem cells into cardiomyocytes.

Technical Data for Zebularine

M. Wt	228.2
Formula	C₉H₁₂N₂O₅
Storage	Desiccate at RT
Purity	≥99% (HPLC)
CAS Number	3690-10-6
PubChem ID	100016
InChi Key	RPQZTTQVRYEKCR-WCTZXXKLSA-N
Smiles	O[C@@H]1[C@@H](CO)O[C@@H](N2C(N=CC=C2)=O)[C@@H]1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Zebularine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	22.82	100
	DMSO	22.82	100

Preparing Stock Solutions for Zebularine

The following data is based on the product molecular weight 228.2.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.38 mL	21.91 mL	43.82 mL
5 mM	0.88 mL	4.38 mL	8.76 mL
10 mM	0.44 mL	2.19 mL	4.38 mL
50 mM	0.09 mL	0.44 mL	0.88 mL

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Reconstitution Calculator

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References for Zebularine

References are publications that support the biological activity of the product.

Ben-Kasus Metabolic activation of zebularine, a novel DNA methylation inhibitor, in human bladder carcinoma cells. Biochem.Pharmacol. 2005 PMID: 15885659
Naeem DNA methylation inhibitors, 5-azacytidine and zebularine potentiate the trans-differentiation of rat bone marrow mesenchymal stem cells into cardiomyocytes. Cardiovasc.Ther. [Epub ahead of print]
Laliberte Potent inhibitors for the deamination of cytosine arabinoside and 5-aza-2'-deoxycytidine by human cytidine deaminase. Cancer Chemother.Pharmacol. 1992 PMID: 1375134