Zymosan, TLR2 Ligand
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26233
Key Product Details
Species
Human
Applications
Functional Assay, Inhibition Activity
Product Summary for Zymosan, TLR2 Ligand
Zymosan is a yeast cell wall derivative that is composed of b-glucans, mannans, mannoproteins and chitin. Zymosan is a known activator of TLR2, of which inflammatory responses to this particle are synergistically generated by collaboration with Dectin-1 and result in NF-kB-induced inflammatory cytokine production and phagocytosis1. Zymosan is also known to directly bind TLR2. Product Validation The data shows that Zymosan activated the TLR2 cells but not the control cells.
Product Specifications
Applications
Functional (Inhibition) (reported in scientific literature (PMID 25846753))
In vitro assay (reported in scientific literature (PMID 27351455))
In vivo assay (reported in scientific literature (PMID 25846753))
Ligand Activation (reported in scientific literature (PMID 25957979))
In vitro assay (reported in scientific literature (PMID 27351455))
In vivo assay (reported in scientific literature (PMID 25846753))
Ligand Activation (reported in scientific literature (PMID 25957979))
Application Notes
This product is useful for Activation of Toll-like Receptor 2 (TLR2).Stimulation of TLR2 has been reported with 1-10 ug/ml. Note: TLR2 activation at this range of concentrations of Zymosan has also been observed in our product validation tests, please see the image.
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 25957979)
Scientific Data Images for Zymosan, TLR2 Ligand
Zymosan, TLR2 Ligand [NBP2-26233] - Ligand activity of Zymosan. The NF-kB/SEAPorter/HEK 293 and TLR2/NFkB/SEAPorter/ HEK 293 cell line cell lines were plated in a 96-well plate at 5 x 10^4 cells/well for 16 h. Cells were then stimulated with Zymosan for 24h. The cell culture supernatants were analyzed for SEAP activity using SEAPorter Assay Kit.
Formulation, Preparation, and Storage
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Reconstitution Instructions
To prepare a 50mg/ml stock solution, mix 50mg Zymosan with 1ml ethanol in a sterile non-pyrogenic vial by vortexing. For further dilution, use sterilized endotoxin-free water or PBS. The stock solution is insoluble and remains opaque. Vortex before use.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -20C in powder form. Store at -80C once reconstituted.
Background: Zymosan, TLR2 Ligand
Alternate Names
Zymosan, TLR2 Ligand
Additional Zymosan, TLR2 Ligand Products
Product Documents for Zymosan, TLR2 Ligand
Product Specific Notices for Zymosan, TLR2 Ligand
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
Loading...
Loading...
Loading...
Loading...