Rapid DAB Staining Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18633
Key Product Details
Applications
Immunohistochemistry
Kit Type
Staining Kit (Colorimetric)
Product Summary for Rapid DAB Staining Kit (Colorimetric)
Rapid DAB Staining Kit (Colorimetric) is a powerful intensification system that significantly enhances chromogenic signals. This system can be integrated into standard immunohistochemistry (IHC) staining methods.
Rapid DAB Staining Kit (Colorimetric) is an enzyme-mediated reaction that utilizes horseradish peroxidase (HRP) to catalyze the deposition of two separate Linkers applied sequentially onto tissue sections or cell preparation. The deposited Linkers can be detected with a streptavidin-peroxidase conjugate followed by a reaction with a peroxidase substrate/chromogen solution such as diaminobenzidine (DAB).
Rapid DAB Staining Kit (Colorimetric) results in a significant increase in sensitivity compared to standard IHC detection methods, while maintaining similar specificity.
Rapid DAB Staining Kit (Colorimetric) is an enzyme-mediated reaction that utilizes horseradish peroxidase (HRP) to catalyze the deposition of two separate Linkers applied sequentially onto tissue sections or cell preparation. The deposited Linkers can be detected with a streptavidin-peroxidase conjugate followed by a reaction with a peroxidase substrate/chromogen solution such as diaminobenzidine (DAB).
Rapid DAB Staining Kit (Colorimetric) results in a significant increase in sensitivity compared to standard IHC detection methods, while maintaining similar specificity.
Scientific Data Images for Rapid DAB Staining Kit (Colorimetric)
Immunohistochemistry: Rapid DAB Staining Kit (Colorimetric) [NBP3-18633]
Immunohistochemistry: Rapid DAB Staining Kit (Colorimetric) [NBP3-18633] - Detection of Ace 2 in human kidney by staining it for 5 min at room temperature with 5 ug/mL of mouse anti-human ACE-2 antibody (MAB9335) using Rapid DAB Staining Kit (NBP3-18633). Tissue was counterstained with Hematoxylin.Immunohistochemistry: Rapid DAB Staining Kit (Colorimetric) [NBP3-18633]
Immunohistochemistry: Rapid DAB Staining Kit (Colorimetric) [NBP3-18633] - Detection of TIM-1 in human kidney by staining it for 5 min at room temperature with 5 ug/mL of rabbit anti-human TIM-1 antibody (MAB17504) using Rapid DAB Staining Kit (NBP3-18633). Tissue was counterstained with Hematoxylin.Immunohistochemistry: Rapid DAB Staining Kit (Colorimetric) [NBP3-18633]
Immunohistochemistry: Rapid DAB Staining Kit (Colorimetric) [NBP3-18633] - Detection of Cathepsin L in human kidney by staining it for 5 min at room temperature with 5 ug/mL of rat anti-human Cathepsin L antibody (MAB9521) using Rapid DAB Staining Kit (NBP3-18633). Tissue was counterstained with Hematoxylin.Kit Contents for Rapid DAB Staining Kit (Colorimetric)
- Empty mixing bottle for Stable DAB/Plus
- Linker 1
- Linker 2
- Stable DAB/Plus Buffer
- Stable DAB/Plus Chromogen
- Tissue Primer
- Tracer
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C in the dark.
Background: Rapid DAB
Additional Rapid DAB Products
Product Documents for Rapid DAB Staining Kit (Colorimetric)
Product Specific Notices for Rapid DAB Staining Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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