VisUBlock Mouse on Mouse Blocking Reagent

Blocking reagent for use with primary antibodies from mouse host and mouse tissue.

Reagents Provided

1mL VisUBlock^™ Mouse on Mouse Blocking Reagent

Stability and Storage

Store at 2 ^°C to 8 ^°C. DO NOT FREEZE.

Precaution

See MSDS.

Reagent Preparation

Mix 30 µL of VisUBlock^™ Mouse on Mouse Blocking Reagent with 1mL of preferred buffer such as PBS or TBS. This will produce approximately 30 mL of working solution, which will stain 100-250 tissue sections.

Procedure

1. Prior to primary antibody incubation, apply Mouse on Mouse IgG Blocking Reagent to tissue section. Incubate 1 hour at room temperature.
2. Tap off, do not rinse, and continue with primary antibody incubation and detection antibody incubation according to protocol set in lab.

Limitations

• FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
• Results may vary due to variations in tissue staining conditions.