Human EMT 3-Color Immunocytochemistry Kit

To assess epithelial to mesenchymal transition (EMT) status in human cells using single-step ICC.

Why Assess EMT Status in Human Cells Using Established Markers?

Epithelial to mesenchymal transition (EMT) is centrally important during embryonic development and is implicated in tissue fibrosis and cancer cell metastasis. The ability to quickly and reliably assess EMT status is critical for developing a greater understanding of the mechanisms that regulate EMT.

The downregulation of epithelial markers, including E-Cadherin, and upregulation of mesenchymal markers, including Snail and Vimentin, can be used to assess EMT status.

Assessing EMT status using 3-color ICC:

• Increases confidence in EMT status by combining 3 different fluorochrome-conjugated antibodies for simultaneous staining.
• Allows researchers to evaluate changes in relative protein levels and subcellular localization of EMT markers.
• Ensures efficient use of time and reagents via single-step staining.

This kit contains the following fluorochrome-conjugated antibodies to analyze EMT status in human cells:

• NL557-conjugated Goat Anti-Human Snail
• NL637-conjugated Goat Anti-Human E-Cadherin
• NL493-conjugated Goat Anti-Human Vimentin

Each antibody is supplied as a 10X stock; enough for 25 assays when used in a 50 µL staining volume per assay.

Stability and Storage

Reagents are stable for 12 months from date of receipt when stored in the dark at 2^° C to 8^°.

Epithelial to Mesenchymal Transition (EMT) is a biological process which is centrally important to embryogenesis and organ development. Epithelial are highly ordered monolayers of cells with a uniform morphology. Cells within epithelial are characterized by the fact that they are adhered tightly to each other. In contrast, mesenchymal cells differ in shape and display an increased capacity for migration and invasion. This change in phenotype is thought to be involved in some oncogenic pathways. Epithelial to mesenchymal transition allows benign tumors to progress into metastatic cancers that can invade other tissues. EMT is also involved in fibrosis during scar tissue development and may be pathologically relevant to the development of progressive fibrotic diseases. Molecular markers of epithelial to mesenchymal transition include increased expression of N-Cadherin and Vimentin, nuclear localization of beta-catenin, and augmented levels of transcription factors that reduce E-Cadherin expression (i.e. Snail1).