Human Pluripotent Stem Cell 3-Color Immunocytochemistry Kit

To verify pluripotency in human stem cells using single-step ICC.

Why Verify the Pluripotency of a Human Stem Cell Population?

Maintaining and expanding healthy, undifferentiated stem cells in culture can be challenging if spontaneous differentiation occurs.

In addition, research studies using embryonic or induced pluripotent stem cells most often require weeks of cell culture before an experimental hypothesis can be tested.

Verifying stem cell pluripotency using 3-color ICC:

• Defines the starting stem cell population.
• Increases confidence in pluripotent status by combining 3 different fluorochrome-conjugated antibodies for simultaneous staining.
• Reduces variability within an experiment and between studies.
• Allows researchers to expand and study high quality, undifferentiated stem cell starting populations.
• Single-step staining ensures efficient use of time and reagents.

This kit contains the following fluorochrome-conjugated antibodies to verify human stem cell pluripotency:

• NL557-conjugated Goat Anti-Human SOX2
• NL637-conjugated Goat Anti-Human Oct-3/4
• NL493-conjugated Goat Anti-Human Nanog

Each antibody is supplied as a 10X stock; enough for 25 assays when used in a 50 µL staining volume per assay.

Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of pre-implantation embryos. Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. In this capacity, pluripotent stem cells have widespread clinical potential for the treatments of heart disease, diabetes, spinal cord injury, and a variety of neurodegenerative disorders.

R&D Systems offers a wide range of products to support pluripotent stem cell culture and differentiation. Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. We also offer defined culture media, which are specifically optimized for use with human or rodent pluripotent stem cells. In addition, R&D Systems offers a variety of products to assess differentiation status and identify specific stem cell types of interest, including panels of marker antibodies, primer pairs, multi-color flow cytometry kits, and specialized verification kits.