2A3

Name: 2A3
Brand: Tocris Bioscience
SKU: 7376

Tocris Bioscience, part of Bio-Techne | Catalog # 7376

Citations (3)

COA / SDS

Reagent for RNA SHAPE-MaP experiments in vivo; cell permeable

Key Product Details

Description:	Reagent for RNA SHAPE-MaP experiments in vivo; cell permeable
Chemical Name:	(2-Aminopyridin-3-yl)(1H-imidazol-1-yl)methanone
Molecular Weight:	188.19

View Full Technical Details for 2A3

Technical Data
Citations

Biological Activity for 2A3

2A3 is an electrophilic chemical probe that acylates the 2'-OH in the RNA backbone. 2A3 can be used for RNA SHAPE-MaP experiments in cell lines or live bacteria. It labels single-stranded or flexible regions more than folded regions (SHAPE methodology) and enables analysis of RNA secondary structures at single nucleotide resolution. For intracellular experiments, 2A3 has higher signal-to-noise ratio and prediction accuracy than NAI (Cat. No. 7004). 2A3 is cell permeable, and can probe both gram-positive and gram-negative bacteria.

Licensing Information

Sold with kind permission of Dr Danny Incarnato

Technical Data for 2A3

M. Wt	188.19
Formula	C₉H₈N₄O
Storage	Store at -20°C
CAS Number	2765091-45-8
InChi Key	HTSCLDQAVWRITQ-UHFFFAOYSA-N
Smiles	O=C(N1C=NC=C1)C2=CC=CN=C2N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 2A3

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	18.82	100

Preparing Stock Solutions for 2A3

The following data is based on the product molecular weight 188.19.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	5.31 mL	26.57 mL	53.14 mL
5 mM	1.06 mL	5.31 mL	10.63 mL
10 mM	0.53 mL	2.66 mL	5.31 mL
50 mM	0.11 mL	0.53 mL	1.06 mL

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References for 2A3

References are publications that support the biological activity of the product.

Marinus A novel SHAPE reagent enables the analysis of RNA structure in living cells with unprecedented accuracy. Nucleic Acids Res. 2021 PMID: 33398343

2A3