Cholesterol

Name: Cholesterol
Brand: Tocris Bioscience
SKU: 7945

Tocris Bioscience, part of Bio-Techne | Catalog # 7945

Citations (16)

COA / SDS

Component of lipid nanoparticles (LNPs); modulator of lipid bilayer fluidity

Key Product Details

Description:	Component of lipid nanoparticles (LNPs); modulator of lipid bilayer fluidity
Chemical Name:	(3β)-Cholest-5-en-3-ol
Purity:	≥95% (HPLC)
Molecular Weight:	386.65

View Full Technical Details for Cholesterol

Technical Data
Citations

Biological Activity for Cholesterol

Cholesterol is a major sterol in all mammalian organisms; it is an essential component that makes up about 20-25% of the structural lipids of cell membranes. Cholesterol determines the permeability and fluidity characteristics of the cell membranes. Cholesterol is an endogenous ligand for estrogen-related receptor α (ERRα). It is also a precursor in several biosynthetic pathways, including steroid hormones and the active form of vitamin D. Cholesterol is a component of lipid nanoparticles (LNPs), and is important in modulating membrane stability, with the effect being context dependent. When combined with lipids that have low gel-liquid crystalline phase transition temperature (T_m), Cholesterol reduces membrane fluidity and increases bilayer thickness; when combined with high T_m lipids, it increases membrane fluidity and narrows the bilayer thickness.

For more information on how to prepare MC3 lipid nanoparticles for RNA delivery, please see our protocol.

Technical Data for Cholesterol

M. Wt	386.65
Formula	C₂₇H₄₆O
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	57-88-5
PubChem ID	5997
InChi Key	HVYWMOMLDIMFJA-DPAQBDIFSA-N
Smiles	C[C@@]12[C@]([C@]3([C@@]([C@]4(C)C(=CC3)C[C@@H](O)CC4)(CC1)[H])[H])(CC[C@@]2([C@@H](CCCC(C)C)C)[H])[H]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Cholesterol

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	19.33	50 with gentle warming
	chloroform	38.66	100

Preparing Stock Solutions for Cholesterol

The following data is based on the product molecular weight 386.65.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.59 mL	12.93 mL	25.86 mL
5 mM	0.52 mL	2.59 mL	5.17 mL
10 mM	0.26 mL	1.29 mL	2.59 mL
50 mM	0.05 mL	0.26 mL	0.52 mL

Molarity Calculator

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Reconstitution Calculator

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References for Cholesterol

References are publications that support the biological activity of the product.

Cheng Dendrimer-based lipid nanoparticles deliver therapeutic fah mRNA to normalize liver function and extend survival in a mouse model of hepatorenal tyrosinemia type I. Adv.Mater 2018 PMID: 30368954
Ghanbari Cholesterol as an endogenous ligand of ERRα promotes ERRα-mediated cellular proliferation and metabolic target gene expression in breast cancer cells. Cell 2020 PMID: 32717915
Tenchov Lipid nanoparticles - from liposomes to mRNA vaccine delivery, a landscape of research diversity and advancement. ACS Nano 2021 PMID: 34181394