Exosome Standards (Human Urine)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49840
Key Product Details
Applications
ELISA, Flow Cytometry, Microscopy, N/A, Western Blot
Product Summary for Exosome Standards (Human Urine)
Highly pure, lyophilized exosome standards with superior stability, optimal for multiple applications including: Assay calibration, Spike-in control for exosome quantification, Protein marker analysis for different techniques such as Western Blot and Flow Cytometry, Extraction and analysis of exosomal RNA and DNA.
Quantity per vial of 30 ug size (number of particles in 30 ug: > 1x10^8). Quantity per vial of 100 ug size (number of particles in 100 ug: > 1x10^10).
Quantity per vial of 30 ug size (number of particles in 30 ug: > 1x10^8). Quantity per vial of 100 ug size (number of particles in 100 ug: > 1x10^10).
Scientific Data Images for Exosome Standards (Human Urine)
![Western Blot: Exosome Standards (Human Urine) [NBP2-49840]](https://resources.bio-techne.com/images/products/Exosome-Standards-Human-Urine-Western-Blot-NBP2-49840-img0001.jpg "Western Blot: Exosome Standards (Human Urine) [NBP2-49840]")
Western Blot: Exosome Standards (Human Urine) [NBP2-49840]
Western Blot: Exosome Standards (Human Urine) [NBP2-49840] - Marker detection by Western Blotting. Reconstituted Exosomes can be directly lysed in Laemmli buffer, then loaded on the Electrophoresis gel. Recommended quantity: 10-20 ug per line.![Nucleic Acid Extraction: Exosome Standards (Human Urine) [NBP2-49840]](https://resources.bio-techne.com/images/products/Exosome-Standards-Human-Urine-Nucleic-Acid-Extraction-NBP2-49840-img0002.jpg "Nucleic Acid Extraction: Exosome Standards (Human Urine) [NBP2-49840]")
Nucleic Acid Extraction: Exosome Standards (Human Urine) [NBP2-49840]
Nucleic Acid Extraction: Exosome Standards (Human Urine) [NBP2-49840] - Profiling of Exosome associated nucleic acids. Reconstituted Exosomes can be used for profiling nucleic acids (RNAs and DNA) biomarkers. The lysis buffer can be added directly to the Exosome preparation. Recommended quantity: 20-50 ug perreaction.Formulation, Preparation, and Storage
Preparation Method
Isolation involves Tangential flow filtration combined with Size Exclusion Chromatogrpahy. Exosomes (small EVs) are quantified and validated for protein content and particle number by Nanoparticle Tracking Analysis as well as for common tetraspanins maker validation. Lyophilization does not alter stability of exosome proteins and nucleic acids.
Formulation
Lyophilized from human urine
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Reconstitution Instructions
Add deionized water, 100 ul for Standard 100 ug and 30 ul for Standard 30 ug, to get a final concentration of 1 mg/mL. Resuspend exosomes pipetting the solution up and down 10-15 times, avoiding bubbles. Vortex the reconstituted standard for 60 seconds.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C. After reconstitution store at -70C.
Background: Exosome Standards (Human Urine)
Additional Exosome Standards (Human Urine) Products
Product Documents for Exosome Standards (Human Urine)
Product Specific Notices for Exosome Standards (Human Urine)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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